Yang Min, Tao Liang, Zhao Cun-Chao, Wang Zi-Lin, Yu Zhi-Jin, Zhou Wen, Wen Yan-Long, Li Ling-Fei, Tian Yang, Sheng Jun
College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.
Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, China.
Front Nutr. 2022 Feb 22;9:824525. doi: 10.3389/fnut.2022.824525. eCollection 2022.
Fatigue is a common physiological phenomenon caused by many complicated factors. Excessive fatigue will lead to a series of uncomfortable reactions and damage body health. leaves (PNL) is a new resource food that good for soothing nerves, nourishing the heart, and strengthening the spleen. Microbial fermentation could increase the content of bio-ingredients and produce new active ingredients. However, the effect of fermented leaves (FPNL) on antifatigue and the molecular mechanisms remain to be elucidated. Thus, in this study, we evaluated the antifatigue effect of co-fermented leaves by and and , and its mechanism was further elucidated. The results showed that FPNL exhibited higher saponins, organic phenolic acids content, and antioxidant activity than PNL. FPNL improved ISO-induced H9c2 myocardial cell damage by alleviating apoptosis (modulating Bax and Bcl-2 protein expression) and reducing antioxidant activity . Moreover, experiment showed that FPNL significantly prolonged the weight-loading swimming time of mice. After gavaged FPNL, the levels of liver glycogen (LG) and serum lactate dehydrogenase (LDH) activity were increased in mice. In contrast, the levels of blood urea nitrogen (BUN), lactate acid, and malondialdehyde (MDA) were decreased. In summary, our results indicated that FPNL showed a good antifatigue effect and .
疲劳是一种由多种复杂因素引起的常见生理现象。过度疲劳会导致一系列不适反应并损害身体健康。[叶子名称未明确，暂用“叶子”代替]（PNL）是一种新型资源食品，具有安神、养心、健脾的功效。微生物发酵可以增加生物成分的含量并产生新的活性成分。然而，发酵[叶子名称未明确，暂用“叶子”代替]（FPNL）的抗疲劳作用及其分子机制仍有待阐明。因此，在本研究中，我们评估了[两种微生物名称未明确，暂用“和”代替]与[一种微生物名称未明确，暂用“和”代替]共同发酵[叶子名称未明确，暂用“叶子”代替]的抗疲劳效果，并进一步阐明其机制。结果表明，FPNL的皂苷、有机酚酸含量和抗氧化活性均高于PNL。FPNL通过减轻细胞凋亡（调节Bax和Bcl-2蛋白表达）和降低抗氧化活性改善了ISO诱导的H9c2心肌细胞损伤。此外，[实验名称未明确，暂用“实验”代替]表明FPNL显著延长了小鼠负重游泳时间。给小鼠灌胃FPNL后，小鼠肝脏糖原（LG）水平和血清乳酸脱氢酶（LDH）活性升高。相反，血尿素氮（BUN）、乳酸和丙二醛（MDA）水平降低。综上所述，我们的结果表明FPNL显示出良好的抗疲劳效果[和相关作用未明确，暂用“和”代替]。
