孕烯醇酮在Y-1小鼠肾上腺细胞中的代谢：高效液相色谱分析及气相色谱-质谱联用对代谢产物的鉴定

Pregnenolone metabolism in Y-1 mouse adrenal cells: HPLC analysis and identification of metabolites by gas chromatography-mass spectrometry.

作者信息

Matsuguchi M, Dehennin L, Habrioux G, Matsuguchi-Moreau L, Degrelle H

机构信息

INSERM, Hôpital St. Vincent de Paul, Paris, France.

出版信息

J Steroid Biochem. 1987 Sep;28(3):311-5. doi: 10.1016/0022-4731(87)91024-7.

DOI:10.1016/0022-4731(87)91024-7

PMID:3657153

Abstract

The Y-1 mouse adrenocortical tumor cells secrete into the culture medium some metabolites of pregnenolone. The long term basic steroid secretion was studied for 24 h by high performance liquid chromatography (HPLC). After 6 h incubation, only one chromatographic peak was shown to be quantitatively important. When the cells were incubated with tritium labeled pregnenolone and carrier, the analysis of radioactivity of each HPLC fraction indicated a rapid incorporation of the exogenous precursor and successive secretion/reabsorption kinetics of the metabolites. The final product, accumulated in the medium, was characterized by gas chromatography-mass spectrometry as 11 beta,20 alpha-dihydroxy-4-pregnen-3-one.

摘要

Y-1小鼠肾上腺皮质肿瘤细胞向培养基中分泌一些孕烯醇酮的代谢产物。通过高效液相色谱法（HPLC）对长期基础类固醇分泌进行了24小时的研究。孵育6小时后，仅一个色谱峰显示在定量上具有重要意义。当细胞与氚标记的孕烯醇酮和载体一起孵育时，对每个HPLC馏分的放射性分析表明外源前体的快速掺入以及代谢产物的连续分泌/重吸收动力学。在培养基中积累的最终产物通过气相色谱-质谱法表征为11β,20α-二羟基-4-孕烯-3-酮。