Department of Dermatology, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea.
Lasers Surg Med. 2023 Feb;55(2):190-199. doi: 10.1002/lsm.23623. Epub 2022 Dec 27.
Application of the picosecond laser in the field of dermatology has expanded from tattoo removal to skin rejuvenation on a clinical basis. Although various mechanisms of pigment removal have been elucidated, the molecular changes associated with skin rejuvenation have yet to be identified. The aim of this study was to explore the theoretical basis and to evaluate the efficacy of skin rejuvenation using a 1064-nm fractional picosecond laser in a mouse model.
We conducted an in vivo study using a fractional picosecond laser on the skin of old and young female hairless mice and performed topographical, histological, micro-, and electron microscopic assessments.
The topography of the skin surface was enhanced and showed increased dermal thickness on histological examination. Electron microscopy revealed disarranged collagen bundles with microspaces and vascular leakage in the upper dermis. Levels of collagen synthesis markers and various inflammatory cytokines, such as procollagens, interleukin-1β, tumor necrosis factor-α, and heat shock proteins, were elevated in the laser-treated skin.
This study provides a possible mechanism for the skin rejuvenation effect of fractional picosecond laser that has been reported previously in clinical observations. Based on our findings, the fractional picosecond laser could be widely applied in clinical settings where dermal regeneration and promotion of skin rejuvenation is required.
皮秒激光在皮肤科的应用已从纹身去除扩展到临床皮肤年轻化。虽然已经阐明了各种去除色素的机制，但与皮肤年轻化相关的分子变化尚未确定。本研究旨在探讨使用 1064nm 分束皮秒激光在小鼠模型中进行皮肤年轻化的理论基础和疗效。
我们在老年和年轻雌性无毛小鼠的皮肤进行了一项分束皮秒激光的体内研究，并进行了形貌学、组织学、微观和电子显微镜评估。
皮肤表面形貌得到改善，组织学检查显示真皮厚度增加。电子显微镜显示真皮上层的胶原纤维束排列紊乱，存在微空间和血管渗漏。激光处理皮肤中的胶原蛋白合成标志物和各种炎症细胞因子（如前胶原蛋白、白细胞介素-1β、肿瘤坏死因子-α和热休克蛋白）水平升高。
本研究为分束皮秒激光的皮肤年轻化效果提供了一种可能的机制，这在之前的临床观察中已有报道。基于我们的发现，分束皮秒激光可以广泛应用于需要真皮再生和促进皮肤年轻化的临床环境中。
