在无细胞体系中氧张力、巯基化合物及血清对梅毒螺旋体（Nichols株）运动性和毒力的影响
Influence of oxygen tension, sulfhydryl compounds, and serum on the motility and virulence of Treponema pallidum (Nichols strain) in a cell-free system.
作者信息
Norris S J, Miller J N, Sykes J A, Fitzgerald T J
出版信息
Infect Immun. 1978 Dec;22(3):689-97. doi: 10.1128/iai.22.3.689-697.1978.
Abstract
The motility and virulence of Treponema pallidum (Nichols strain) were monitored during incubation in a modified tissue culture medium to study the effects of oxygen tension and medium composition on survival of the organism. A basal medium of Eagle minimal essential medium with 50% fresh, heat-inactivated normal rabbit serum was used inasmuch as better survival occurred with 50% normal rabbit serum than with lower concentrations. Addition of 0.5 to 2.0 mM dithiothreitol or 2.0 mM dithioerythritol to the basal medium led to significantly longer retention of T. pallidum viability in the presence of 3% oxygen than under aerobic or anaerobic conditions. The results of this investigation lend support to the classification of T. pallidum as a microaerophilic organism and provide direction for the design of potentially successful culture systems, with or without tissue culture cells.
摘要
在改良组织培养基中培养期间，对梅毒螺旋体（Nichols株）的运动性和毒力进行了监测，以研究氧张力和培养基成分对该生物体存活的影响。使用含有50%新鲜、热灭活正常兔血清的Eagle最低必需培养基作为基础培养基，因为50%正常兔血清比低浓度血清能使梅毒螺旋体有更好的存活情况。向基础培养基中添加0.5至2.0 mM二硫苏糖醇或2.0 mM二硫赤藓糖醇，在3%氧气存在的情况下，梅毒螺旋体的活力保留时间比在需氧或厌氧条件下显著延长。本研究结果支持将梅毒螺旋体归类为微需氧菌，并为设计潜在成功的培养系统提供了方向，无论有无组织培养细胞。
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Influence of oxygen tension, sulfhydryl compounds, and serum on the motility and virulence of Treponema pallidum (Nichols strain) in a cell-free system.在无细胞体系中氧张力、巯基化合物及血清对梅毒螺旋体（Nichols株）运动性和毒力的影响
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