Hohmann A, Schmidt G, Rowley D
Infect Immun. 1979 Jul;25(1):27-33. doi: 10.1128/iai.25.1.27-33.1979.
After oral feeding of mice with avirulent Salmonella, Escherichia coli, or hybrid strains, only certain bacterial strains were able to multiply and persist within the small intestinal Peyer's patches. After oral vaccination alone, or oral priming and subsequent parenteral boosting, antibody class and titers were detected, using a radioimmunoassay on serum and intestinal fluid or a plaque-forming cell assay on spleens. Only those strains that persisted in the Peyer's patches stimulated the production of serum and intestinal immunoglobulin A antibodies against their respective O antigens. Nonpersistent strains were weakly immunogenic, and antibodies, when present, were largely non-immunoglobulin A and confined to the serum.
用无毒力的沙门氏菌、大肠杆菌或杂交菌株经口喂养小鼠后，只有某些细菌菌株能够在小肠派尔集合淋巴结内繁殖并持续存在。单独经口接种疫苗，或经口初免随后进行肠外加强免疫后，使用血清和肠液的放射免疫测定法或脾脏的空斑形成细胞测定法检测抗体类别和滴度。只有那些在派尔集合淋巴结中持续存在的菌株能刺激产生针对其各自O抗原的血清和肠道免疫球蛋白A抗体。非持续性菌株免疫原性较弱，即便有抗体产生，大多也不是免疫球蛋白A，且局限于血清中。
