用于评估马胰岛素失调的碳水化合物丸。

Carbohydrate pellets to assess insulin dysregulation in horses.

机构信息

Faculty of Science, Queensland University of Technology, Brisbane, Queensland, Australia.

Boehringer Ingelheim Vetmedica GmbH, Ingelheim, Germany.

出版信息

J Vet Intern Med. 2023 Jan;37(1):302-314. doi: 10.1111/jvim.16621. Epub 2022 Dec 30.

DOI:10.1111/jvim.16621

PMID:36583553

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9889680/

Abstract

BACKGROUND

A glycemic challenge test is used for the diagnosis of insulin dysregulation (ID) in horses and ponies. Different forms of the test exist where the administrative route and dose of glucose vary, which makes interpretation of results challenging.

HYPOTHESIS/OBJECTIVES: To evaluate the palatability of, and blood glucose and insulin responses to, carbohydrate pellets fed as an oral glucose test (OGT), and to establish the diagnostic threshold for ID when using the pellets.

ANIMALS

University and privately-owned horses and ponies (n = 157) comprised of 31 breeds and both sexes.

METHODS

Multicenter cohort study. A custom-produced glycemic pellet was offered for free intake at 0.5 g/kg BW soluble carbohydrate and serum insulin and blood glucose concentrations measured before and after (60, 120, and 180 minutes) the pellets were offered. Pellet acceptance and intake time (those that finished within 10 minutes) were determined to assess palatability.

RESULTS

The pellets were palatable to 132/157 animals, and ponies found the pellets more (P = .004) palatable than horses. The median intake time (4 [3-6] minutes) was positively correlated with acceptance grade (r = .51; P < .0001). Consumption of the pellets elicited peak blood glucose (6.6 [5.8-7.8] mmol/L) and serum insulin (40.5 [19-99.8] μIU/mL) responses at 120 minutes. At 120 minutes the optimal cut-off was 83 μIU/mL (95% CI: 70-99 μIU/mL) for the IMMULITE 2000XPi assay.

CONCLUSIONS AND CLINICAL IMPORTANCE

The pellets were palatable and a suitable, novel carbohydrate source for the OGT.

摘要

背景

血糖挑战试验用于诊断马和小型马的胰岛素失调（ID）。该试验有不同的形式，其给药途径和葡萄糖剂量有所不同，这使得结果的解释具有挑战性。

假设/目的：评估口服葡萄糖试验（OGT）中使用的碳水化合物丸的适口性以及血糖和胰岛素反应，并确定使用该丸剂诊断 ID 的诊断阈值。

动物

来自 31 个品种的大学和私人拥有的马和小型马（n=157），包括两性。

方法

多中心队列研究。以 0.5 g/kg BW 可溶碳水化合物的剂量免费提供一种定制生产的血糖丸，在提供丸剂前后（60、120 和 180 分钟）测量血清胰岛素和血糖浓度。通过确定接受率和摄入时间（10 分钟内完成的那些）来评估适口性。

结果

157 匹马和小型马中有 132 种接受了丸剂，并且与马相比，小型马发现丸剂更（P=0.004）可口。摄入时间的中位数（4 [3-6] 分钟）与接受等级呈正相关（r=0.51；P<.0001）。摄入丸剂可引起血糖（6.6 [5.8-7.8] mmol/L）和血清胰岛素（40.5 [19-99.8] μIU/mL）在 120 分钟时达到峰值。在 120 分钟时，对于 IMMULITE 2000XPi 检测，最佳截断值为 83 μIU/mL（95%CI：70-99 μIU/mL）。