Department of Pathology, The Third Hospital of Nanchang, Nanchang City, Jiangxi, China.
BMC Womens Health. 2022 Dec 30;22(1):559. doi: 10.1186/s12905-022-02151-9.
To investigate the positive rate and clinical applicability of liquid-based fungal method for detecting of vaginal fungi. We collect the secretions from the posterior vaginal fornix and the vaginal wall of 198 patients with clinically suspected fungi vaginitis patients for study.
The vaginal fungi of vaginal discharge were detected by fluorescence method, i.e., by liquid-based thin-layer fungi fluorescence morphology staining detection kit (liquid-based fungal method), saline smear method and fungal culture method.
The positive rate of liquid-based fungal method, saline smear method was 50%, 25.75% respectively. The positive rate of liquid-based fungal method were 50%. The true positive rate of liquid-based fungal method (87.85%) was higher than that of saline smear method (45.79%, P < 0.001), which was easy to miss diagnosis. Moreover, the Kappa (K) of liquid-based fungal method was 0.81, and P < 0.01, which was statistically significant, indicating that the consistency of the two detection methods is good. Of the eight common symptoms of fungal vaginitis, the positive symptom coincidence rate of liquid-based fungal method was consistent with that of fungal culture method. It was also easier to see fungi under a microscope than with saline smear method.
The liquid-based fungal method has a high positive coincidence rate and accuracy in the detection of vaginal fungi, and it is convenient to operate and implement steps. Therefore, it may be applied in clinical practice. Or a combination of several detection methods can be used.
为了研究液基真菌法检测阴道真菌的阳性率和临床适用性。我们收集了 198 例临床疑似真菌性阴道炎患者的后阴道穹窿和阴道壁分泌物进行研究。
采用荧光法检测阴道分泌物中的阴道真菌，即采用液基薄层真菌荧光形态染色检测试剂盒（液基真菌法）、生理盐水涂片法和真菌培养法。
液基真菌法、生理盐水涂片法的阳性率分别为 50%、25.75%。液基真菌法的阳性率为 50%。液基真菌法的真阳性率（87.85%）高于生理盐水涂片法（45.79%，P<0.001），容易漏诊。此外，液基真菌法的 Kappa（K）值为 0.81，P<0.01，差异有统计学意义，表明两种检测方法的一致性较好。在真菌性阴道炎的 8 种常见症状中，液基真菌法的阳性症状符合率与真菌培养法一致。与生理盐水涂片法相比，用液基真菌法更容易在显微镜下看到真菌。
液基真菌法在检测阴道真菌方面具有较高的阳性符合率和准确性，操作方便，易于实施。因此，它可能适用于临床实践。或者可以结合几种检测方法。
