Xie Wenjia, Chen Junyu, Cheng Xinting, Feng Hao, Zhang Xin, Zhu Zhou, Dong Shanshan, Wan Qianbing, Pei Xibo, Wang Jian
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, 310006, China.
Small. 2023 Apr;19(14):e2205941. doi: 10.1002/smll.202205941. Epub 2023 Jan 1.
Drug-resistant bacterial infection impairs tissue regeneration and is a challenging clinical problem. Metal-organic frameworks (MOFs)-based photodynamic therapy (PDT) opens up a new era for antibiotic-free infection treatment. However, the MOF-based PDT normally encounters limited photon absorbance under visible light and notorious recombination of photogenerated holes and electrons, which significantly impede their applications. Herein, a MOFs-based nanosystem (AgNPs@MOFs) with enhanced visible light response and charge carrier separation is developed by modifying MOFs with silver nanoparticles (AgNPs) to improve PDT efficiency. The AgNPs@MOFs with enhanced photodynamic performance under visible light irradiation mainly disrupt bacteria translation process and the metabolism of purine and pyrimidine. In addition, the introduction of AgNPs endows nanosystems with chemotherapy ability, which causes destructive effect on bacterial cell membrane, including membrane ATPase protein and fatty acids. AgNPs@MOFs show excellent synergistic drug-resistant bacterial killing efficiency through multiple mechanisms, which further restrain bacterial resistance. In addition, biocompatible AgNPs@MOFs pose potential tissue regeneration ability in both Methicillin-resistant Staphylococcus aureus (MRSA)-related soft and hard tissue infection. Overall, this study provides a promising perspective in the exploration of AgNPs@MOFs as nano antibacterial medicine against drug-resistant bacteria for infected tissue regeneration in the future.
耐药细菌感染会损害组织再生，是一个具有挑战性的临床问题。基于金属有机框架（MOF）的光动力疗法（PDT）为无抗生素感染治疗开辟了一个新时代。然而，基于MOF的PDT通常在可见光下遇到有限的光子吸收以及光生空穴和电子的严重复合问题，这显著阻碍了它们的应用。在此，通过用银纳米颗粒（AgNP）修饰MOF来开发具有增强的可见光响应和电荷载流子分离的基于MOF的纳米系统（AgNPs@MOFs），以提高PDT效率。在可见光照射下具有增强光动力性能的AgNPs@MOFs主要破坏细菌的翻译过程以及嘌呤和嘧啶的代谢。此外，AgNP的引入赋予纳米系统化疗能力，这会对细菌细胞膜造成破坏作用，包括膜ATP酶蛋白和脂肪酸。AgNPs@MOFs通过多种机制显示出优异的协同抗耐药细菌杀灭效率，进一步抑制细菌耐药性。此外，生物相容性良好的AgNPs@MOFs在耐甲氧西林金黄色葡萄球菌（MRSA）相关的软组织和硬组织感染中都具有潜在的组织再生能力。总体而言，本研究为未来探索将AgNPs@MOFs作为抗耐药细菌的纳米抗菌药物用于感染组织再生提供了一个有前景的视角。
