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乌鲁苏-阿马雷拉蜂蜜的成熟过程():宏基因组学、核磁共振氢代谢组学、物理化学性质和抗氧化特性

Maturation of honey from Uruçú-Amarela (): Metagenomics, metabolomics by NMR H, physicochemical and antioxidant properties.

作者信息

Silva José Renato, Henrique-Bana Fernanda Carla, Villas-Bôas Jerônimo Kahn, Colombo Pimentel Tatiana, Spinosa Wilma Aparecida, Prudencio Sandra Helena

机构信息

Department of Food Science and Technology, Center of Agricultural Sciences, State University of Londrina, Londrina-PR 86057-970, Brazil.

Reenvolver, Ribeirão Preto-SP 14095-190, Brazil.

出版信息

Food Chem (Oxf). 2022 Dec 13;6:100157. doi: 10.1016/j.fochms.2022.100157. eCollection 2023 Jul 30.

Abstract

The objective of this study was to characterize the microbiota biodiversity of Uruçú-Amarela honey through metagenomics. Furthermore, the impact of maturation temperatures (20 and 30 °C) and time (0-180 days) on the physicochemical and antioxidant properties was investigated. H NMR was performed to verify metabolites formed during maturation. Uruçú-Amarela honey was mainly composed by lactic acid bacteria and osmophilic yeasts of genus . Maturation at 30 °C led to a higher fermentation activity, resulting in greater carbohydrate consumption, ethanol formation (0.0-0.6 %) and increased acidity (34.78-45.74 meq/kg) over the 180 days. It also resulted in honey with higher brown color (a* 0.7 to 3.89, b* 17.50-25.29) and antioxidant capacity, corroborating that the maturation is a suitable preservation technique for stingless bee honey, because it does not cause negative changes as it extends the shelf life of the stingless bee honey.

摘要

本研究的目的是通过宏基因组学表征乌鲁库-阿马雷拉蜂蜜的微生物群落生物多样性。此外,还研究了成熟温度(20和30°C)和时间(0 - 180天)对其理化性质和抗氧化特性的影响。采用核磁共振氢谱(¹H NMR)来验证成熟过程中形成的代谢产物。乌鲁库-阿马雷拉蜂蜜主要由乳酸菌和特定属的嗜渗酵母组成。在30°C下成熟导致更高的发酵活性,在180天内导致更大的碳水化合物消耗、乙醇形成(0.0 - 0.6%)和酸度增加(34.78 - 45.74 meq/kg)。它还使蜂蜜具有更深的褐色(a* 0.7至3.89,b* 17.50 - 25.29)和抗氧化能力,证实成熟是一种适合无刺蜂蜂蜜的保存技术,因为它在延长无刺蜂蜂蜜保质期的同时不会引起负面变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3210/9794890/0a48c31009e5/gr1.jpg

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