China Jiliang University, College of Life Sciences, Engineering Training Centre/College of Innovation, Hangzhou 310018, China.
Zhejiang Institute of Economics and Trade, Hangzhou 310018, China.
Oxid Med Cell Longev. 2022 Dec 22;2022:1759009. doi: 10.1155/2022/1759009. eCollection 2022.
AdoR-1, the single adenosine receptor homolog in , which belongs to the superfamily of G-protein coupled receptors (GPCRs), mediates most of the physiological effects of extracellular adenosine. Adenosine has been proved to improve the survival rate of in oxidative stress conditions. However, the potential mechanism of adenosine's protective effect against oxidative stress via AdoR-1 has not been studied. In this study, were divided into three groups: two groups with paraquat treatment, one in the presence and one in the absence of adenosine, and an untreated control group. Results indicate that many differentially expressed genes were found to be enriched significantly in neural-related signaling pathways among transcriptome data of three groups. Further gene network analysis showed that some important genes well known to be involved in promoting the acetylcholine release pathway, such as , , and and those involved in promoting the neuropeptide release pathway, such as were upregulated by paraquat induction but downregulated after adenosine treatment. Meanwhile, a completely opposite trend was observed for the gene that inhibits the acetylcholine-release and neuropeptide-release pathway. Additionally, some biochemical assays including SOD, GSSG, GSH, and AChE were measured to identify the potential protection of adenosine against oxidative stress between wild-type strain N2 and gene knockout strain EG6890. Conclusively, our study revealed series of adenosine receptor-mediated genes in that might act as regulators of paraquat-induced oxidative stress and may indicate adenosine's promising protective effects.
AdoR-1 是秀丽隐杆线虫中唯一的腺苷受体同源物，属于 G 蛋白偶联受体 (GPCR) 超家族，介导细胞外腺苷的大多数生理作用。已有研究证明，腺苷可提高 在氧化应激条件下的存活率。然而，腺苷通过 AdoR-1 发挥其抗氧化应激保护作用的潜在机制尚未得到研究。在本研究中， 将分为三组：两组用百草枯处理，一组有腺苷存在，一组没有腺苷，还有一组未处理的对照组。结果表明，在三组的转录组数据中，发现许多差异表达的基因在神经相关信号通路中显著富集。进一步的基因网络分析表明，一些重要的基因，如已知参与促进乙酰胆碱释放途径的基因 、 、 和 ，以及参与促进神经肽释放途径的基因 ，在百草枯诱导下上调，但在用腺苷处理后下调。同时， 基因表现出完全相反的趋势， 基因抑制乙酰胆碱释放和神经肽释放途径。此外，还进行了一些生化测定，包括 SOD、GSSG、GSH 和 AChE，以鉴定腺苷对野生型 N2 菌株和 基因敲除 EG6890 菌株氧化应激的潜在保护作用。综上所述，本研究揭示了秀丽隐杆线虫中一系列可能作为百草枯诱导氧化应激调节剂的腺苷受体介导基因，并表明腺苷具有有希望的保护作用。
