Aguilar-Díaz Hugo, Quiroz-Castañeda Rosa Estela, Salazar-Morales Karina, Miranda-Miranda Estefan
Unidad de Artropodología del Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Carretera Federal Cuernavaca-Cuautla No. 8534, Progreso, 62550, Jiutepec, Morelos, Mexico.
Unidad de Anaplasmosis del Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Carretera Federal Cuernavaca-Cuautla No. 8534, Progreso, 62550, Jiutepec, Morelos, Mexico.
Curr Res Parasitol Vector Borne Dis. 2021 Dec 7;2:100066. doi: 10.1016/j.crpvbd.2021.100066. eCollection 2022.
Ticks are hematophagous ectoparasites with importance to animal and human health. In recent years, the study of ticks has had significant development, including immune response, vector-host interactions, physiological and multi-omics approaches. However, one of the main impediments is obtaining a significant amount of high-quality hemolymph. For this reason, we developed a protocol that allows obtaining up to 100 μl of hemolymph free of host blood per engorged tick. The technique consists of continuous hipocuticular punctures of the tick dorsum and an anticoagulant buffer that impedes hemolymph coagulation, allowing constant extravasation and ensuring high yields. Additionally, the hemocytes recovered with this protocol are intact and can be used for further analysis. The high-quality hemolymph obtained using this protocol and its applications will help to better understand the processes involving the hemolymph and its components. Although there are other hemolymph extraction protocols, the method developed here is very well suited for , and in our experience, results in better yields and high-quality samples.
蜱是对动物和人类健康具有重要影响的吸血性体外寄生虫。近年来，蜱的研究取得了显著进展，包括免疫反应、媒介-宿主相互作用、生理学和多组学方法。然而，主要障碍之一是获取大量高质量的血淋巴。因此，我们开发了一种方案，每只饱血蜱最多可获取100微升不含宿主血液的血淋巴。该技术包括对蜱背部进行连续的角质下穿刺以及一种抗凝缓冲液，该缓冲液可阻止血淋巴凝固，使血淋巴持续渗出并确保高产率。此外，通过该方案回收的血细胞是完整的，可用于进一步分析。使用该方案获得的高质量血淋巴及其应用将有助于更好地理解涉及血淋巴及其成分的过程。尽管有其他血淋巴提取方案，但此处开发的方法非常适合，并且根据我们的经验，可产生更高的产量和高质量的样本。
