Suwito Widodo, Nugroho Widagdo Sri, Adji Rahmat Setya, Andriani Andriani, Kusumaningtyas Eny, Martini Tri
Research Center Processing Food Technology, National Research Innovation Innovation Agency (BRIN), Gunungkidul 55861, Yogyakarta, Indonesia.
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Sleman 55281, Yogyakarta, Indonesia.
Vet World. 2022 Nov;15(11):2587-2592. doi: 10.14202/vetworld.2022.2587-2592. Epub 2022 Nov 15.
Subclinical mastitis (SCM) in Etawah-grade (PE) goats in Yogyakarta, Indonesia, is commonly due to . At present, from SCM in PE goats in Yogyakarta has not been characterized. Therefore, this study aimed to phenotypically characterize , which has been isolated from SCM of PE goats.
A total of 314 lactating PE goats were collected from 60 PE goat farms (e.g., Sleman, Bantul, and Kulonprogo) located in parts of Yogyakarta with an average age of 3-4 years old, three of which showed SCM based on the California mastitis test (CMT). Subclinical mastitis is confirmed in PE goats if CMT shows ++ or +++. Furthermore, was detected by biochemical assays. could determine hemolysin (Hae), coagulase (Coa), clumping factor (Cf), and antibiotic susceptibility. Hemolytic bacteria were detected by culturing on blood agar plate, and Cf was detected by slide agglutination. The production of Coa was detected by tube coagulation. susceptibility was determined by antimicrobial agar diffusion using a paper disc.
Phenotypically characterized from PE goats with SCM in Yogyakarta, Indonesia, Coa-, Cf-, and Hae- were found to be resistant to erythromycin (ERYTHRO), ampicillin (AMP), penicillin (PEN-G), and sulfamethoxazole (SULFA).
The phenotypic characteristic of , which was obtained from SCM in PE goats in Yogyakarta, consists of Coa, and Cf-. cannot perform hemolysis of red blood cells. This phenotypic characteristic can prevent and control SCM in PE goats. Several antibiotics such as ERYTHRO, AMP, PEN-G, and SULFA were no longer effective for treating SCM in PE goats because has developed its resistance to these antibiotics.
印度尼西亚日惹埃塔瓦级（PE）山羊的亚临床乳腺炎（SCM）通常是由……引起的。目前，日惹PE山羊亚临床乳腺炎的……尚未得到表征。因此，本研究旨在从表型上表征从PE山羊亚临床乳腺炎中分离出的……。
从位于日惹部分地区的60个PE山羊养殖场（如斯莱曼、班图尔和库隆普罗戈）收集了总共314只泌乳期PE山羊，平均年龄为3 - 4岁，其中三只根据加利福尼亚乳腺炎检测（CMT）显示患有亚临床乳腺炎。如果CMT显示++或+++，则在PE山羊中确认亚临床乳腺炎。此外，通过生化分析检测……。……可以确定溶血素（Hae）、凝固酶（Coa）、聚集因子（Cf）和抗生素敏感性。通过在血琼脂平板上培养检测溶血细菌，通过玻片凝集检测Cf。通过试管凝固检测Coa的产生。使用纸片通过抗菌琼脂扩散法测定……敏感性。
在印度尼西亚日惹患有亚临床乳腺炎的PE山羊中从表型上表征的……，发现Coa -、Cf -和Hae -对红霉素（ERYTHRO）、氨苄青霉素（AMP）、青霉素（PEN - G）和磺胺甲恶唑（SULFA）耐药。
从日惹PE山羊亚临床乳腺炎中获得的……的表型特征包括Coa -和Cf -。……不能对红细胞进行溶血。这种表型特征可以预防和控制PE山羊的亚临床乳腺炎。几种抗生素如ERYTHRO、AMP、PEN - G和SULFA对治疗PE山羊的亚临床乳腺炎不再有效，因为……已对这些抗生素产生耐药性。
