Liu Donghao, Yi Simin, Ni Xu, Zhang Jingjing, Wang Fangqi, Yang Ping, Liu Meiru, Peng Jun, Dramou Pierre, He Hua
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 211198, P. R. China.
Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, 211198, P. R. China.
Chempluschem. 2023 Jan;88(1):e202200286. doi: 10.1002/cplu.202200286.
Nanozymes have advantages over natural enzymes in terms of efficiency, stability, and economy. MVSM (Mixed Valence State MOF) is a nano-oxidase with uricase-like activity that may catalyze uric acid (UA) in the body into allantoin and H O to treat gout and hyperuricemia by substituting natural uricase. However, it cannot specifically identify and choose UA. To increase the selectivity and affinity of MVSM for UA, the composite material MVSM@MIP is innovatively synthesized using a new synthetic approach termed the "two-step synthesis method," which may prevent UA from being oxidized by MVSM during manufacture in this study. At the same time, this study also provides experimental proof of the effective creation of the material, the advantages of the "two-step synthesis approach," and the high selectivity and affinity of MVSM@MIP for UA. Based on these findings, the suggested technique may be used to effectively catalyze uric acid in human urine with high activity.
纳米酶在效率、稳定性和经济性方面优于天然酶。MVSM（混合价态金属有机框架）是一种具有尿酸酶样活性的纳米氧化酶，它可以通过替代天然尿酸酶将体内的尿酸（UA）催化为尿囊素和H₂O，以治疗痛风和高尿酸血症。然而，它不能特异性地识别和选择UA。为了提高MVSM对UA的选择性和亲和力，采用一种称为“两步合成法”的新合成方法创新性地合成了复合材料MVSM@MIP，这可以在本研究的制造过程中防止UA被MVSM氧化。同时，本研究还提供了该材料有效制备、“两步合成法”的优势以及MVSM@MIP对UA具有高选择性和亲和力的实验证据。基于这些发现，所建议的技术可用于高效催化人尿液中的尿酸。
