Key Laboratory of Longevity and Aging-Related Diseases of Chinese Ministry of Education, Guangxi Colleges and Universities, Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Medical University, Nanning, 530021, Guangxi, People's Republic of China.
Department of Clinical Laboratory, The Fourth People's Hospital of Nanning, Guangxi AIDS Clinical Treatment Center (Nanning), No. 1 Erli, Changgang Road, Nanning, 530023, Guangxi, People's Republic of China.
Sci Rep. 2023 Jan 2;13(1):53. doi: 10.1038/s41598-022-26396-4.
Selenium is an essential micronutrient element. For the extremely biotoxic of selenite, Selenium nanoparticles (SeNPs) is gaining increasing interest. In this work, a selenium-enriched strain with highly selenite-resistant (up to 173 mmol/L) was isolated from the local specialty food of longevity area and identified as Paenibacillus motobuensis (P. motobuensis) LY5201. Most of the SeNPs were accumulated extracellular. SeNPs were around spherical with a diameter of approximately 100 nm. The X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy showed that the purified SeNPs consisted of selenium and proteins. Our results suggested that P. motobuensis LY5201could be a suitable and robust biocatalyst for SeNPs synthesis. In addition, the cytotoxicity effect and the anti-invasive activity of SeNPs on the HepG2 showed an inhibitory effect on HepG2, indicating that SeNPs could be used as a potential anticancer drug.
硒是一种必需的微量元素。由于亚硒酸盐的毒性极高，因此人们对硒纳米颗粒（SeNPs）越来越感兴趣。在这项工作中，从长寿地区的当地特色食品中分离出一株具有高抗亚硒酸盐能力（高达 173mmol/L）的富硒菌株，并鉴定为 Motobuensis 芽孢杆菌（P. motobuensis）LY5201。大部分 SeNPs 积累在细胞外。SeNPs 呈球形，直径约为 100nm。X 射线光电子能谱和傅里叶变换红外光谱表明，纯化的 SeNPs 由硒和蛋白质组成。我们的结果表明，Motobuensis 芽孢杆菌 LY5201 可以作为合成 SeNPs 的合适且强大的生物催化剂。此外，SeNPs 对 HepG2 的细胞毒性作用和抗侵袭活性表明对 HepG2 具有抑制作用，表明 SeNPs 可用作潜在的抗癌药物。
