多重显色免疫组织化学染色和分析来自于小鼠或人类的石蜡组织切片。

Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human.

机构信息

Barts Cancer Institute, Queen Mary University of London, John Vane Science Building, Charterhouse Square, London EC1M 6BQ, UK.

出版信息

STAR Protoc. 2022 Dec 16;3(4):101879. doi: 10.1016/j.xpro.2022.101879. Epub 2022 Dec 2.

DOI:10.1016/j.xpro.2022.101879

PMID:36595909

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9720345/

Abstract

Here we describe a multiplex chromogenic immunohistochemistry platform to stain and analyze two markers in paraffin tissue sections from mouse or human. The basis of the protocol is a series of stripping and re-probing steps with subsequent image analysis, which allows the user to perform multiplex imaging in a reliable and affordable manner. Here, we describe specific usage to assess the levels of PD-L1 in tumor-associated macrophages. We have used different antibodies and assessed this protocol for up to five consecutive antibodies per slide. For complete details on the use and execution of this protocol, please refer to Orgaz et al. (2020)..

摘要

在这里，我们描述了一种多重显色免疫组化平台，可以对来自小鼠或人类的石蜡组织切片中的两种标志物进行染色和分析。该方案的基础是一系列洗脱和重新探测步骤，随后进行图像分析，这使得用户能够以可靠和经济的方式进行多重成像。在这里，我们描述了特定的用途，以评估肿瘤相关巨噬细胞中 PD-L1 的水平。我们已经使用了不同的抗体，并评估了该方案在每张载玻片上最多连续使用五种抗体的情况。有关此方案的使用和执行的完整详细信息，请参阅 Orgaz 等人。（2020）。