Tiwari Menka, Singh Rajat, Jha Rintu, Singh Prashant
Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, India.
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
Front Plant Sci. 2022 Dec 16;13:1050765. doi: 10.3389/fpls.2022.1050765. eCollection 2022.
Crop plants encounter a variety of biotic challenges in the field and faces significant reduction in crop yield. In the current scenario of an ever increasing global population, there is an urgent need to protect plant health by using sustainable approach to maximize the crop productivity and to mitigate the food demands. Nowadays, we mostly rely on chemical crop protection techniques, which are causing a number of environmental and health difficulties. Defence priming is a chemical-free, eco-friendly, and sustainable strategy of crop protection, which is also called "green vaccination. In the present study, for the first time, we used as a priming agent to protect wheat crop from spot blotch disease. We have established -mediated defence priming in wheat against for sustainable crop improvement. We have characterised the morphological, disease phenotype, biochemical and yield parameters of -primed and non-primed wheat under disease pressure. -primed plants were found to be more protected against as compared to non-primed plants. Biochemical studies indicated that there is no direct defence response after priming stimulus but the defence response was activated only after triggering stimulus in terms of enhanced defence metabolites in primed plants as compared to non-primed plants. In the present study, since defence was activated only when required, that is under disease pressure, there was no unnecessary allocation of resources towards defence. Hence, no yield penalty was shown in primed plants as compared to control. We further evaluated the inheritance of primed state to the next generation and found that progeny of primed parents also performed better than progeny of non-primed parents under disease pressure in terms of protection from as well as yield performance. This strategy has the potential to protect crop without any yield penalty and causing environmental degradation. Our research findings indicate that -mediated defence priming could be an alternative approach for improving wheat productivity under biotic stress. To be our best knowledge, this is the first documented report for the mediated defence priming and induced inheritance in wheat plant. This study will open new arenas in sustainable crop protection strategies for the exploitation of defence priming in crop plants.
农作物在田间会遇到各种生物挑战，导致作物产量大幅下降。在全球人口不断增长的当前形势下，迫切需要采用可持续的方法来保护植物健康，以最大限度地提高作物生产力并缓解粮食需求。如今，我们主要依赖化学作物保护技术，这引发了许多环境和健康问题。防御引发是一种无化学物质、环保且可持续的作物保护策略，也被称为“绿色疫苗接种”。在本研究中，我们首次使用[具体物质]作为引发剂来保护小麦作物免受叶斑病侵害。我们已经在小麦中建立了[具体物质]介导的针对[病原体]的防御引发，以实现可持续的作物改良。我们对在病害压力下经[具体物质]引发和未经引发的小麦的形态、病害表型、生化和产量参数进行了表征。结果发现，与未经引发的植物相比，经[具体物质]引发的植物对[病原体]的抵抗力更强。生化研究表明，引发刺激后没有直接的防御反应，但与未经引发的植物相比，引发植物在触发刺激后，即受到病害压力时，由于防御代谢产物的增加，防御反应才被激活。在本研究中，由于防御仅在需要时，即在病害压力下才被激活，因此没有不必要的资源用于防御。因此，与对照相比，引发植物没有表现出产量损失。我们进一步评估了引发状态向下一代的遗传情况，发现引发亲本的后代在病害压力下，在抵御[病原体]以及产量表现方面也比未引发亲本的后代表现更好。这种策略有可能在不造成任何产量损失和环境退化的情况下保护作物。我们的研究结果表明，[具体物质]介导的防御引发可能是在生物胁迫下提高小麦生产力的一种替代方法。据我们所知，这是关于小麦植株中介导防御引发和诱导遗传的第一份有记录的报告。这项研究将为可持续作物保护策略开辟新领域，以便在作物中利用防御引发。
