Department of Ophthalmology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Department of Physiology, Inner Mongolia Medical University, Inner Mongolia, China.
Mol Vis. 2022 Dec 21;28:432-440. eCollection 2022.
We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application.
HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. HADSCs were intraocularly injected into oxygen-induced retinopathy (OIR) mice to check the movement, fusion, proliferation, and prognosis in vivo. Electron microscopy was used to check retinal changes to confirm the safety of hADSCs in intraocular application.
After induction, hADSCs expressed von Willebrand Factor (vWF), the cell marker of ECs. The hADSCs were distributed above the retina after an intravitreal injection in the OIR mice. The injected cells did not fuse with the retina and gathered in the central and peripheral areas, which is the lesion area of the OIR model. Five days after the hADSC intravitreal injection, the area of neovascularization was reduced by 94.83% compared with that of the OIR group. Hematologic staining and electron microscopy did not show noticeable proliferation and degeneration of the retina.
This study provides evidence for the intraocular application of hADSCs.
我们设计了一项研究，旨在寻找人脂肪间充质干细胞（hADSCs）在眼内应用中的诱导、迁移、融合、增殖和安全性的理论依据。
诱导 hADSCs 以确认其在体外能够表达内皮细胞（ECs）的特征。将 hADSCs 眼内注射到氧诱导的视网膜病变（OIR）小鼠中，以检查体内的迁移、融合、增殖和预后。电子显微镜用于检查视网膜变化，以确认 hADSCs 在眼内应用的安全性。
诱导后，hADSCs 表达了血管性血友病因子（vWF），这是 ECs 的细胞标志物。在 OIR 小鼠的玻璃体内注射后，hADSCs 分布在视网膜上方。注射的细胞没有与视网膜融合，聚集在中央和周边区域，这是 OIR 模型的病变区域。hADSC 玻璃体内注射后 5 天，与 OIR 组相比，新生血管化面积减少了 94.83%。血液学染色和电子显微镜检查均未显示出明显的视网膜增殖和变性。
本研究为 hADSCs 的眼内应用提供了证据。
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