School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong, China.
Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China.
Anal Chem. 2022 Sep 20;94(37):12836-12844. doi: 10.1021/acs.analchem.2c02798. Epub 2022 Sep 5.
Hydrazine is widely used in industrial and agricultural production, but excessive hydrazine possesses a serious threat to human health and environment. Here two new ratiometric fluorescence probes, and , with the hydroxyl coumarin chalcone unit as the sensing site are developed, which can achieve colorimetric and ratiometric recognition for hydrazine with good sensitivity, excellent selectivity, and anti-interference. The calculated fluorescence limits of detections are 0.26 μM () and 0.14 μM (). The ratiometric fluorescence response to hydrazine is realized through the adjustment of donor and receptor units in coumarin conjugate structure terminals, accompanied by fluorescence peak shift about 200 nm (, 188 nm; , 229 nm). Stronger electropositivity in the carbon-carbon double bond is helpful to the first phase addition reaction between the probe and hydrazine. Higher phenol activity in the hydroxyl coumarin moiety will facilitate the following dihydro-pyrazole cyclization reaction. In addition, both of these probes realized the convenient detection of hydrazine vapor. The probes were also successfully applied to detect hydrazine in actual water samples, different soils, and living cells.
水合肼广泛应用于工农业生产，但过量的水合肼对人类健康和环境构成严重威胁。本文设计了两个以羟基香豆素查尔酮单元为传感位点的新型比率荧光探针 和 ，可以对水合肼进行高灵敏度、高选择性、抗干扰的比色和比率识别。计算出的荧光检测限分别为 0.26 μM () 和 0.14 μM (). 香豆素共轭结构末端供体和受体单元的调节实现了对水合肼的比率荧光响应，同时伴有荧光峰约 200nm 的移动 (, 188nm;, 229nm). 碳碳双键上更强的正电性有助于探针与水合肼的第一步加成反应。羟基香豆素部分更高的酚活性将有利于随后的二氢吡唑环化反应。此外，这两种探针都实现了对水合肼蒸气的便捷检测。该探针还成功应用于实际水样、不同土壤和活细胞中肼的检测。
