一种香豆素融合的“开-关”型荧光探针，用于高选择性检测水合肼。
A coumarin-fused 'off-on' fluorescent probe for highly selective detection of hydrazine.
机构信息
College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.
College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; Key Laboratory of Chemical Biology of Hebei Province, Hebei University, Baoding 071002, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Apr 5;230:118075. doi: 10.1016/j.saa.2020.118075. Epub 2020 Jan 16.
Hydrazine is a kind of widely used industrial raw material and a toxic biochemical reagent. Due to its toxic to organisms, hydrazine has been classified to be a hazardous environmental pollutant. It is urgent to develop fluorescent probe tools for selective sensitivity detection of hydrazine in the environment and the body. We developed here a new coumarin-based fluorescent probe for hydrazine detection. The probe can selectively detect hydrazine over other environmental and endogenous interfering analytes with a large off-on fluorescence response. The detection limit is 8.55 ppb, which is well below the allowed threshold limit value. The sensing mechanism is hydrazine-induced pyrazole ring formation, which is confirmed by HRMS and DFT calculation methods. Additionally, the probe could also be applied for hydrazine imaging in living HeLa cells.
水合肼是一种用途广泛的工业原料和有毒生化试剂。由于其对生物具有毒性，水合肼已被归类为有害的环境污染物。因此，开发用于选择性、灵敏检测环境和生物体内水合肼的荧光探针工具迫在眉睫。我们在此开发了一种新的基于香豆素的荧光探针，用于水合肼的检测。该探针可以选择性地检测水合肼，而对其他环境和内源性干扰分析物具有较大的荧光响应。检测限为 8.55ppb，远低于允许的阈值。传感机制是水合肼诱导吡唑环形成，这通过高分辨率质谱（HRMS）和密度泛函理论（DFT）计算方法得到了证实。此外，该探针还可用于活 HeLa 细胞中水合肼的成像。
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