Ranjbari Sara, Rezayi Majid, Arefinia Reza, Aghaee-Bakhtiari Seyed Hamid, Hatamluyi Behnaz, Pasdar Alireza
Chemical Engineering Department, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Talanta. 2023 Apr 1;255:124247. doi: 10.1016/j.talanta.2022.124247. Epub 2022 Dec 31.
Cancer is one of the leading causes of death worldwide and a crisis for global health. Breast cancer is the second most common cancer globally. In the perusal, a novel electrochemical biosensor amplified with hierarchical flower-like gold, poly (n-butyl acrylate), and MXene (AuHFGNs/PnBA-MXene) nanocomposite and activated by highly special antisense ssDNA (single-stranded DNA) provide a promising alternative for miRNA-122 detection as a biomarker of breast cancer. The biosensor presented a detection limit of 0.0035 aM (S/N = 3) with a linear range from 0.01 aM to 10 nM. The platform was tried on 20 breast cancer miRNAs extracted from actual serum specimens (10 positives and 10 negatives). Founded on the quantitatively obtained outcomes and statistic analysis (t-test, box-graph, receiver performance characteristic curve, and cut-off amount), the biosensor showed a meaningful discrepancy between the native and positive groups with 100% specificity and 100% sensitivity. While, RT-qPCR showed less specificity and sensitivity (70% specificity, 100% sensitivity) than the proposed biosensor. To assess the quantitative capacity and biosensor detection limit for clinical tests, the biosensor diagnosis performance for continually diluted miRNA extracted from patients was compared to that gained by RT-qPCR results, indicating that the biosensor detection limit was lower than RT-qPCR. ssDNA/AuHFGN/PnBA-MXene/GCE displayed little cross-reaction with other sequences and also showed desirable stability, reproducibility, and specificity and stayed stable until 32 days. As a result, the designed biosensor can perform as a hopeful method for diagnosis applications.
癌症是全球主要死因之一，也是全球健康面临的危机。乳腺癌是全球第二大常见癌症。在这项研究中，一种新型电化学生物传感器，用分级花状金、聚（丙烯酸正丁酯）和MXene（AuHFGNs/PnBA-MXene）纳米复合材料进行放大，并由高度特异性的反义单链DNA（ssDNA）激活，为作为乳腺癌生物标志物的miRNA-122检测提供了一种有前景的替代方法。该生物传感器的检测限为0.0035 aM（S/N = 3），线性范围为0.01 aM至10 nM。该平台对从实际血清标本中提取的20种乳腺癌miRNA进行了测试（10个阳性和10个阴性）。基于定量获得的结果和统计分析（t检验、箱线图、接收器性能特征曲线和截断量），该生物传感器在天然组和阳性组之间显示出显著差异，特异性为100%，灵敏度为100%。同时，RT-qPCR显示出的特异性和灵敏度（特异性70%，灵敏度100%）低于所提出的生物传感器。为了评估该生物传感器在临床试验中的定量能力和检测限，将从患者中提取的连续稀释miRNA的生物传感器诊断性能与RT-qPCR结果获得的性能进行了比较，表明该生物传感器的检测限低于RT-qPCR。ssDNA/AuHFGN/PnBA-MXene/GCE与其他序列几乎没有交叉反应，还表现出良好的稳定性、重现性和特异性，并且在32天内保持稳定。因此，所设计的生物传感器可作为一种有希望的诊断应用方法。
