Chemical Engineering Department, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran.
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Mikrochim Acta. 2023 Nov 24;190(12):482. doi: 10.1007/s00604-023-06062-w.
A poly(n-butyl acrylate)-gold-MXene quantum dots (PBA-Au-MXene QD) nanocomposite-based biosensor is presented that is modified by unique antisense single-stranded DNA (ssDNA) and uses the electrochemical detection methods of DPV, CV, and EIS to early detect miR-122 as a breast cancer biomarker in real clinical samples. This fabrication method is based on advanced nanotechnology, at which a poly(n-butyl acrylate) (PBA) as a non-conductive polymer transforms into a conductive composite by incorporating Au-MXene QD. This biosensor had a limit of detection (LOD) of 0.8 zM and a linear range from 0.001 aM to 1000 nM, making it capable of detecting the low concentrations of miR-122 in patient samples. Moreover, it allows approximately 100% sensitivity and 100% specificity for miR-122 without extraction. The synthesis and detection characteristics were evaluated by different complementary tests such as AFM, FTIR, TEM, and FESEM. This new biosensor can have a high potential in clinical applications to detect breast cancer early and hence improve patient outcomes.
一种基于聚（正丁基丙烯酸酯）-金-MXene 量子点（PBA-Au-MXene QD）纳米复合材料的生物传感器被提出，该传感器通过独特的反义单链 DNA（ssDNA）进行修饰，并使用电化学检测方法 DPV、CV 和 EIS 来早期检测 miR-122，作为乳腺癌生物标志物在真实临床样本中。这种制造方法基于先进的纳米技术，其中聚（正丁基丙烯酸酯）（PBA）作为非导电聚合物通过掺入 Au-MXene QD 转化为导电复合材料。该生物传感器的检测限（LOD）为 0.8 zM，线性范围为 0.001 aM 至 1000 nM，使其能够检测患者样本中 miR-122 的低浓度。此外，它无需提取即可实现对 miR-122 的约 100%灵敏度和 100%特异性。通过 AFM、FTIR、TEM 和 FESEM 等不同互补测试评估了合成和检测特性。这种新的生物传感器在临床应用中具有很高的潜力，可以早期检测乳腺癌，从而改善患者的预后。
