Heng H, Rucker R B, Crotty J, Dubick M A
Department of Nutrition, University of California, Davis 95616.
Toxicol Lett. 1987 Oct;38(3):225-37. doi: 10.1016/0378-4274(87)90003-8.
The effects of protein deficiency or food restriction and ozone exposure on lung, heart and liver superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were studied in weanling and adult rats. Two groups of rats were fed diets containing 4 or 16% protein. A third group was fed the 16% protein diet, but at the level consumed by the rats fed the 4% protein diet. After 3 weeks (weanling) or 5 weeks (adult), one-half of the rats in each group were exposed continuously to 0.64 ppm ozone for 7 days. In adult rat lung, O3 exposure typically stimulated Cu,Zn-SOD and GPx activities in all groups, but in weanling rats only GPx activity was elevated and only in rats fed the 16% protein diet. Liver Cu,Zn-SOD activity was also influenced by diet; in adult rats, liver Mn-SOD and GPx activities were often depressed following O3 exposure. Heart SOD and GPx, however, were not affected by ozone or diet. The pulmonary and hepatic effects due to diet and O3 further illustrate the importance of nutritional status when assessing the health effects of O3 exposure.
研究了蛋白质缺乏或食物限制以及臭氧暴露对断奶大鼠和成年大鼠肺、心脏和肝脏中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性的影响。两组大鼠分别喂食含4%或16%蛋白质的饲料。第三组喂食16%蛋白质的饲料，但喂食量与喂食4%蛋白质饲料的大鼠相同。3周（断奶大鼠）或5周（成年大鼠）后，每组一半的大鼠连续7天暴露于0.64 ppm的臭氧中。在成年大鼠肺中，臭氧暴露通常会刺激所有组的铜锌超氧化物歧化酶（Cu,Zn-SOD）和谷胱甘肽过氧化物酶（GPx）活性，但在断奶大鼠中，只有喂食16%蛋白质饲料的大鼠的谷胱甘肽过氧化物酶（GPx）活性升高。肝脏铜锌超氧化物歧化酶（Cu,Zn-SOD）活性也受饮食影响；在成年大鼠中，臭氧暴露后肝脏锰超氧化物歧化酶（Mn-SOD）和谷胱甘肽过氧化物酶（GPx）活性常降低。然而，心脏中的超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）不受臭氧或饮食的影响。饮食和臭氧对肺和肝脏的影响进一步说明了在评估臭氧暴露对健康的影响时营养状况的重要性。
