Department of Radiotherapy, Cancer Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Department of Radiotherapy, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
BMC Cancer. 2023 Jan 6;23(1):20. doi: 10.1186/s12885-022-10492-9.
Tumor-associated neutrophils (TANs) in the tumor microenvironment are prognostic biomarkers in many malignancies. However, it is unclear whether TANs can serve as a prognostic marker for clinical outcomes in patients with glioblastoma (GBM), as classified according to World Health Organization Classification of Tumors of the Central Nervous System, fifth edition (CNS5). In the present study, we analyzed correlations of TANs and peripheral blood neutrophils prior to radiotherapy with overall survival (OS) in GBM (CNS5).
RNA-seq expression profiles of patients with newly diagnosed GBM (CNS5) were extracted from The Cancer Genome Atlas (TCGA), and The Chinese Glioma Genome Atlas (CGGA). TAN infiltration was inferred using CIBERSORTx algorithm. Neutrophil counts prior to radiotherapy in newly diagnosed GBM (CNS5) were obtained from the First Affiliated Hospital of Fujian Medical University. The prognostic value of TANs and peripheral blood neutrophils before radiotherapy was investigated using Kaplan-Meier analysis and Cox proportional hazards models. The robustness of these findings was evaluated by sensitivity analysis, and E values were calculated.
A total of 146 and 173 individuals with GBM (CNS5) were identified from the TCGA and CGGA cohorts, respectively. High infiltration of TANs was of prognostic of poor OS in TCGA (HR = 1.621, 95% CI: 1.004-2.619) and CGGA (HR = 1.546, 95% CI: 1.029-2.323). Levels of peripheral blood neutrophils before radiotherapy (HR = 2.073, 95% CI: 1.077-3.990) were independently associated with poor prognosis. Sensitivity analysis determined that the E-value of high TANs infiltration was 2.140 and 2.465 in the TCGA and CGGA cohorts.
TANs and peripheral blood neutrophil levels before radiotherapy are prognostic of poor outcomes in GBM (CNS5).
肿瘤微环境中的肿瘤相关中性粒细胞（TANs）是许多恶性肿瘤的预后生物标志物。然而，根据世界卫生组织中枢神经系统肿瘤分类第五版（CNS5），TANs 是否可以作为胶质母细胞瘤（GBM）患者临床结局的预后标志物尚不清楚。在本研究中，我们分析了 TANs 和放疗前外周血中性粒细胞与胶质母细胞瘤（CNS5）患者总生存期（OS）的相关性。
从癌症基因组图谱（TCGA）和中国胶质瘤基因组图谱（CGGA）中提取了新诊断的胶质母细胞瘤（CNS5）患者的 RNA-seq 表达谱。使用 CIBERSORTx 算法推断 TAN 浸润。从福建医科大学第一附属医院获得新诊断的胶质母细胞瘤（CNS5）患者放疗前的中性粒细胞计数。使用 Kaplan-Meier 分析和 Cox 比例风险模型研究放疗前 TANs 和外周血中性粒细胞的预后价值。通过敏感性分析和计算 E 值来评估这些发现的稳健性。
从 TCGA 和 CGGA 队列中分别确定了 146 名和 173 名胶质母细胞瘤（CNS5）患者。TAN 浸润程度高与 TCGA（HR=1.621，95%CI：1.004-2.619）和 CGGA（HR=1.546，95%CI：1.029-2.323）的 OS 预后不良相关。放疗前外周血中性粒细胞水平（HR=2.073，95%CI：1.077-3.990）与不良预后独立相关。敏感性分析确定 TCGA 和 CGGA 队列中 TAN 高浸润的 E 值分别为 2.140 和 2.465。
放疗前 TANs 和外周血中性粒细胞水平与 GBM（CNS5）的不良预后相关。
