利用具有高底物耐受性的重组大肠杆菌 HMFOMUT 全细胞，从生物质衍生的呋喃中提高 5-羟甲基-2-糠酸和糠酸的生物合成。
Improved biosynthesis of 5-hydroxymethyl-2-furancarboxylic acid and furoic acid from biomass-derived furans with high substrate tolerance of recombinant Escherichia coli HMFOMUT whole-cells.
机构信息
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, PR China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, PR China; Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, PR China.
出版信息
Bioresour Technol. 2020 May;303:122930. doi: 10.1016/j.biortech.2020.122930. Epub 2020 Jan 29.
The main aim of this work was to firstly develop a selective oxidation approach for biologically converting 5-hydroxymethylfurfural and furfural into the corresponding furan-based carboxylic acids with recombinant Escherichia coli HMFOMUT. Whole-cells of this recombinant strain harbored good biocatalytic activity in a narrow pH range (pH 6.5-7.0), which had high tolerance toward furfural (up to 50 mM) and 5-hydroxymethylfurfural (up to 150 mM), well-known potential inhibitors against microorganisms. 5-Hydroxymethyl-2-furancarboxylic acid and furoic acid could be obtained at 96.9% and 100% yield from 5-hydroxymethylfurfural (150 mM) and furfural (50 mM) at 30 °C and pH 7.0. The improved substrate tolerance of Escherichia coli HMFOMUT is gaining a great interest to synthesize value-added furan-based carboxylic acids, which has potential industrial applications.
本工作的主要目的是首先开发一种选择性氧化方法，利用重组大肠杆菌 HMFOMUT 将 5-羟甲基糠醛和糠醛生物转化为相应的呋喃基羧酸。该重组菌株的全细胞在较窄的 pH 范围（pH 6.5-7.0）内具有良好的生物催化活性，对糠醛（高达 50 mM）和 5-羟甲基糠醛（高达 150 mM）具有高耐受性，这两种物质都是已知的对微生物有潜在抑制作用的物质。在 30°C 和 pH 7.0 下，5-羟甲基-2-呋喃羧酸和呋喃酸可分别从 5-羟甲基糠醛（150 mM）和糠醛（50 mM）中获得 96.9%和 100%的产率。大肠杆菌 HMFOMUT 对底物耐受性的提高对于合成有价值的呋喃基羧酸具有重要意义，具有潜在的工业应用前景。
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