Department of Chemistry, Quaid-Azam University, Islamabad 45320, Pakistan.
School of Applied Sciences and Humanities, National University of Technology (NUTECH), Islamabad 44000, Pakistan.
Molecules. 2022 Dec 21;28(1):49. doi: 10.3390/molecules28010049.
The current work presents a sensitive, selective, cost-effective, and environmentally benign protocol for the detection of ibuprofen (IBP) by an electrochemical probe made of a glassy carbon electrode modified with Ag-ZnO and MWCNTs. Under optimized conditions, the designed sensing platform was found to sense IBP up to a 28 nM limit of detection. The interaction of IBP with bovine serum albumin (BSA) was investigated by differential pulse voltammetry. IBP-BSA binding parameters such as the binding constant and the stoichiometry of complexation were calculated. The results revealed that IBP and BSA form a single strong complex with a binding constant value of 8.7 × 10. To the best of our knowledge, this is the first example that reports not only IBP detection but also its BSA complexation.
目前的工作提出了一种灵敏、选择性好、经济实惠且对环境友好的协议，用于通过由玻璃碳电极修饰的 Ag-ZnO 和 MWCNTs 制成的电化学探针检测布洛芬（IBP）。在优化条件下，发现所设计的传感平台能够检测到低至 28 nM 的 IBP 检测限。通过差分脉冲伏安法研究了 IBP 与牛血清白蛋白（BSA）的相互作用。计算了 IBP-BSA 结合参数，如结合常数和配合物的化学计量比。结果表明，IBP 和 BSA 形成了一个具有 8.7×10 的结合常数值的单一强复合物。据我们所知，这是第一个不仅报道 IBP 检测，而且还报道其与 BSA 络合的例子。
