Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam.
Vietnam Academy of Science and Technology, Graduate University of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam.
Molecules. 2022 Dec 29;28(1):280. doi: 10.3390/molecules28010280.
We present a visual tool and facile method to detect MCF-7 breast cancer cells by using YVO:Eu@silica-NH-GDA-IgG bio-nanocomplexes. To obtain these complexes, YVO:Eu nanoparticles with a uniform size of 10-25 nm have been prepared firstly by the hydrothermal process, followed by surface functionalization to be bio-compatible and conjugated with cancer cells. The YVO:Eu@silica-NH-GDA-IgG nanoparticles exhibited an enhanced red emission at 618 nm under an excitation wavelength of 355 nm and were strongly coupled with MCF-7 breast cancer cells via biological conjugation. These bio-nanocomplexes showed a superior sensitiveness for MCF-7 cancer cell labelling with a detection percentage as high as 82%, while no HEK-293A healthy cells were probed under the same conditions of in vitro experiments. In addition, the detection percentage of MCF-7 breast cancer cells increased significantly via the functionalization and conjugation of YVO:Eu nanoparticles. The experimental results demonstrated that the YVO:Eu@silica-NH-GDA-IgG bio-nanocomplexes can be used as a promising labelling agent for biomedical imaging and diagnostics.
我们提出了一种可视化工具和简便的方法，通过使用 YVO：Eu@silica-NH-GDA-IgG 生物纳米复合物来检测 MCF-7 乳腺癌细胞。为了获得这些复合物，我们首先通过水热法制备了尺寸均匀的 10-25nm 的 YVO：Eu 纳米粒子，然后进行表面功能化使其具有生物相容性，并与癌细胞结合。YVO：Eu@silica-NH-GDA-IgG 纳米粒子在 355nm 激发波长下表现出增强的 618nm 红色发射，并通过生物共轭强烈与 MCF-7 乳腺癌细胞结合。这些生物纳米复合物对 MCF-7 癌细胞标记具有优异的敏感性，检测百分比高达 82%，而在相同的体外实验条件下，未探测到 HEK-293A 健康细胞。此外，通过 YVO：Eu 纳米粒子的功能化和共轭，MCF-7 乳腺癌细胞的检测百分比显著增加。实验结果表明，YVO：Eu@silica-NH-GDA-IgG 生物纳米复合物可用作生物医学成像和诊断的有前途的标记剂。
