一种 6nm 的超稳定光致荧光 DNA FluoroCube，用于荧光成像。
A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging.
机构信息
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.
Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.
出版信息
Nat Methods. 2020 Apr;17(4):437-441. doi: 10.1038/s41592-020-0782-3. Epub 2020 Mar 16.
Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based 'FluoroCubes' that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.
光漂白限制了荧光生物样本的成像。我们开发了基于 DNA 的“FluoroCubes”，其大小与绿色荧光蛋白相似，可单点连接蛋白质，光漂白寿命延长约 54 倍，发射的光子数比单个有机染料多约 43 倍。我们证明 DNA FluoroCubes 是单分子成像的出色工具，可实现对单个肌动蛋白的追踪，其精度可达纳米级，追踪距离超过 800 步。
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