用于 l-酪氨酸特异性识别和可视化检测的分子印迹电纺显色膜。

Molecular imprinted electrospun chromogenic membrane for l-tyrosine specific recognition and visualized detection.

机构信息

College of Chemical Engineering, Fuzhou University, China.

出版信息

Talanta. 2019 Nov 1;204:647-654. doi: 10.1016/j.talanta.2019.06.051. Epub 2019 Jun 15.

DOI:10.1016/j.talanta.2019.06.051

Abstract

In this work, molecularly imprinted electrospun chromogenic membrane(MIP-ECM) was synthesized using P-Hydroxybenzene propanoic acid as imitate template molecule and Poly (vinyl alcohol)(PVA) as substrate. Ninhydrin is used as chromogenic agent for visualizing detection of target molecule l-tyrosine(Tyr). The results showed that molecularly imprinted membrane exhibited selectivity and specific adsorption capacity for l-tyrosine. The influences of environmental factors, such as reaction temperature, the amount of ninhydrin and elution time, on chromogenic properties were examined by SEM and optical microscope. Gray value analysis is proceeded by software Image J. Due to the facilitate preparation and intuitive visualization, the proposed method was a promising candidate for selective recognition of bio-generated acid and amino molecules.

摘要

在这项工作中，以对羟基苯丙酸为模拟模板分子，聚乙烯醇（PVA）为基底，合成了分子印迹电纺显色膜（MIP-ECM）。水合茚三酮被用作显色剂，用于可视化检测目标分子 l-酪氨酸（Tyr）。结果表明，印迹膜对 l-酪氨酸表现出选择性和特异性吸附能力。通过扫描电子显微镜和光学显微镜研究了环境因素，如反应温度、水合茚三酮用量和洗脱时间对显色性能的影响。通过软件 Image J 进行灰度值分析。由于制备简便、可视化直观，该方法有望成为生物衍生酸和氨基酸分子选择性识别的候选方法。