Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Anal Chim Acta. 2022 Mar 22;1199:339574. doi: 10.1016/j.aca.2022.339574. Epub 2022 Feb 4.
Here a highly selective molecular imprinting polymer was developed to attenuate biofilm formation of the multidrug-resistant pathogen Pseudomonas aeruginosa by disrupting the intermolecular signaling system. Firstly, a dummy template molecular imprinting polymer (MIP) was rationally designed through molecular modeling to capture 2-heptyl-3-hydroxy-4-quinolone (Pseudomonas quinolone signal). This multifunctional signaling molecule interferes with the pathogenicity of P. aeruginosa as an auto-inducer. Then, the synthesized MIP and the non-imprinted polymer (NIP) as reference polymer were evaluated for their binding capacity and biofilm inhibition. The results indicated a significant difference in biofilm inhibition (∼56%) between imprinted (∼67%) and non-imprinted (∼11%) polymer, which is an impressive level, especially for the treatment of various surfaces affected by P. aeruginosa. These results open a new window in the special biological application of MIPs as a promising candidate to reduce concerns in clinical or industrial issues by preventing microbial infections.
这里开发了一种高选择性的分子印迹聚合物，通过破坏多药耐药病原体铜绿假单胞菌的分子间信号系统来减弱生物膜的形成。首先，通过分子模拟合理设计了一种虚拟模板分子印迹聚合物 (MIP) 以捕获 2-庚基-3-羟基-4-喹诺酮（铜绿假单胞菌喹诺酮信号）。这种多功能信号分子作为自诱导物干扰铜绿假单胞菌的致病性。然后，评估了合成的 MIP 和非印迹聚合物 (NIP) 作为参考聚合物的结合能力和生物膜抑制作用。结果表明，印迹聚合物（约 67%）和非印迹聚合物（约 11%）之间的生物膜抑制存在显著差异（约 56%），这是一个令人印象深刻的水平，特别是对于治疗受铜绿假单胞菌影响的各种表面。这些结果为 MIP 在特殊生物应用方面开辟了一个新的窗口，作为一种有前途的候选物，可以通过防止微生物感染来减少临床或工业问题中的相关问题。
