从烟草中分离到一种新型杆状病毒的测序和系统发育特征分析。
Sequencing and phylogenetic characterization of a novel Polerovirus from Nicotiana tabacum.
机构信息
Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
出版信息
Virus Genes. 2020 Oct;56(5):657-661. doi: 10.1007/s11262-020-01782-0. Epub 2020 Jul 30.
In this study, we reported the complete genome of a novel Polerovirus, named Tobacco yellow virus (TYV), which can be transmitted by Myzus persicae. TYV had a single-stranded RNA genome of 5735 nucleotides in length and contained six putative open reading frames (ORFs). Phylogenetic analysis with whole genome nucleotide sequences and amino acid sequences deduced from the conserved domain of the RNA-dependent RNA polymerase, clustered TYV with Potato leafroll virus from the genus Polerovirus with high bootstrap values. However, TYV clustered with Brassica yellow virus using amino acid sequences deduced from the conserved domain of the coat protein. Taken together with the identities between ORFs in TYV and related ORFs in species from Polerovirus, our results strongly suggested TYV is a novel species of the genus Polerovirus.
在这项研究中，我们报告了一种新型的 Polerovirus（烟草黄花叶病毒，TYV）的完整基因组，它可以通过桃蚜传播。TYV 的基因组是一个长 5735 个核苷酸的单链 RNA，包含六个假定的开放阅读框（ORFs）。基于 RNA 依赖的 RNA 聚合酶保守结构域推导的全基因组核苷酸序列和氨基酸序列的系统发育分析将 TYV 与 Polerovirus 属的马铃薯卷叶病毒聚为一簇，具有较高的自举值。然而，TYV 与 Brassicacyellowvirus 则是基于衣壳蛋白保守结构域推导的氨基酸序列聚类的。与 TYV 和 Polerovirus 属中相关种的 ORFs 之间的同一性结合起来，我们的结果强烈表明 TYV 是 Polerovirus 属的一个新种。
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