Chiang Mai University, Chiang Mai, Thailand.
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
Arch Razi Inst. 2022 Jun 30;77(3):1281-1289. doi: 10.22092/ARI.2021.356820.1920. eCollection 2022 Jun.
The application of low-cost feed additives as an immunostimulant for different species of fish is an emerging research area in aquaculture. The present study aimed at incorporating a byproduct from coffee processing, coffee silverskin, as an immunostimulant in Nile Tilapia. A total of 225 Tilapia fingerlings (each weighing 23.04±0.07) were fed on diets as follows: Diet 1 (control), Diet 2 (20 gm/kg), Diet 3 (40 gm/kg), Diet 4 (80 gm/kg) and Diet 5 (180 gm/kg) of coffee silverskin. The feeding experiment continued for four weeks; moreover, growth, as well as immunological studies, were carried out by collecting mucus and blood serum from the liver and intestine of the fish. A significant increase in growth performance was observed in fish fed with a 40 gm/kg coffee silverskin-containing diet. The same effect was found in immunological parameters, including complement activity, serum lysozyme activity, and respiratory burst activity. Therefore, it is concluded that the study was definitely a successful one, and a diet with 40 gm/kg of coffee silverskin (Diet 3) was effective, compared to the rest of the compositions.
将低成本饲料添加剂作为免疫增强剂应用于不同种类的鱼类是水产养殖中一个新兴的研究领域。本研究旨在将咖啡加工的副产品——咖啡银皮作为尼罗罗非鱼的免疫增强剂。将 225 尾罗非鱼鱼苗（平均体重为 23.04±0.07 克）分别投喂以下几种饲料：饲料 1（对照组）、饲料 2（20 克/千克）、饲料 3（40 克/千克）、饲料 4（80 克/千克）和饲料 5（180 克/千克）的咖啡银皮。喂养实验持续了四周；此外，通过从鱼的肝脏和肠道收集黏液和血清，进行了生长和免疫学研究。结果发现，投喂含有 40 克/千克咖啡银皮的饲料的鱼的生长性能显著提高。在免疫参数方面也发现了同样的效果，包括补体活性、血清溶菌酶活性和呼吸爆发活性。因此，可以得出结论，本研究是成功的，与其他成分相比，含有 40 克/千克咖啡银皮的饲料（饲料 3）是有效的。
