亚微摩尔浓度的线粒体外钙离子对大鼠肝脏线粒体呼吸速率的刺激作用。
Stimulation of the respiration rate of rat liver mitochondria by sub-micromolar concentrations of extramitochondrial Ca2+.
作者信息
Johnston J D, Brand M D
机构信息
Department of Biochemistry, University of Cambridge, U.K.
出版信息
Biochem J. 1987 Jul 1;245(1):217-22. doi: 10.1042/bj2450217.
Abstract
- The respiration rate of rat liver mitochondria was stimulated by up to 70% when the extramitochondrial Ca2+ concentration was raised from 103 to 820 nM. This occurred when pyruvate, 2-oxoglutarate, or threo-(Ds)-isocitrate was employed as substrate, but not when succinate was used. 2. Ruthenium Red prevented the stimulation of mitochondrial respiration by extramitochondrial Ca2+, showing that the effect required Ca2+ uptake into the mitochondrial matrix. 3. Starvation of rats for 48 h abolished the stimulation of mitochondrial respiration by extramitochondrial Ca2+ when pyruvate was used as substrate, but did not affect the stimulation of 2-oxoglutarate oxidation by extramitochondrial Ca2+. 4. Our findings are in accord with proposals that oxidative metabolism in liver mitochondria may be stimulated by Ca2+ activation of intramitochondrial dehydrogenases.
摘要
- 当线粒体外Ca2+浓度从103 nM升高至820 nM时，大鼠肝线粒体的呼吸速率最多可被刺激70%。当使用丙酮酸、2-氧代戊二酸或苏式-（Ds）-异柠檬酸作为底物时会出现这种情况，但使用琥珀酸时则不会。2. 钌红可阻止线粒体外Ca2+对线粒体呼吸的刺激，表明该效应需要Ca2+摄入线粒体基质。3. 大鼠饥饿48小时后，以丙酮酸作为底物时，线粒体外Ca2+对线粒体呼吸的刺激作用消失，但不影响线粒体外Ca2+对2-氧代戊二酸氧化的刺激作用。4. 我们的研究结果与以下观点一致，即肝线粒体中的氧化代谢可能受线粒体内脱氢酶的Ca2+激活作用所刺激。
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