Dadar Maryam, Brangsch Hanka, Alamian Saeed, Neubauer Heinrich, Wareth Gamal
Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses (IBIZ), Naumburger Str. 96a, 07743 Jena, Germany.
One Health. 2023 Jan 3;16:100483. doi: 10.1016/j.onehlt.2023.100483. eCollection 2023 Jun.
Brucellosis is one of the most common zoonoses in the Middle East. It is causing economic losses to the livestock industry and has a great public health concern. Little is known about the genetic diversity and distribution of brucellae in Iran. Therefore, forty spp. strains ( and ) isolated from animals and humans were analyzed by whole genome sequencing (WGS) technology using single nucleotide polymorphism (SNP) analysis and core genome multilocus sequence typing (cgMLST). isolates were obtained from lymph nodes (cows and camels), milk (cows, camels and sheep), and aborted foetus samples (sheep and goats), as well as cerebrospinal fluid and blood of humans. The isolates were originating from thirteen provinces of Iran and isolated between 2015 and 2020. According to MLST, ST8 and ST2 were the most frequent sequence types in and , respectively. Based on phylogeographic reconstruction using cgSNP analysis, the investigated Iranian strains belonged to the American and Mediterranean lineages of the phylogeny. Furthermore, cgSNP analysis revealed a similarity between Iranian isolates and strains from Iraq and Egypt. Therefore, the origin of the Iranian strains can be suggested to be strains from neighboring and Middle East countries. Moreover, cgMLST analysis showed that the Iranian strains were closely relative to strains recovered from sheep and humans in Iraq, Afghanistan, Syria, Turkmenistan, and Pakistan. In the current panel of strains, cgMLST and cgSNP analysis provided an appropriate and accurate tool for effective traceback analyses for spp. from Iran. The results of cgSNP and cgMLST helped to understand the geographic distribution and interspecies transmission of Iranian strains and highlight the importance of specific brucellosis control measures in Iran with regard to the One-Health approach.
布鲁氏菌病是中东地区最常见的人畜共患病之一。它给畜牧业造成经济损失，并引发了重大的公共卫生问题。人们对伊朗布鲁氏菌的遗传多样性和分布了解甚少。因此，利用单核苷酸多态性（SNP）分析和核心基因组多位点序列分型（cgMLST）技术，通过全基因组测序（WGS）对从动物和人类中分离出的40株布鲁氏菌菌株（羊种布鲁氏菌和牛种布鲁氏菌）进行了分析。分离株取自淋巴结（牛和骆驼）、牛奶（牛、骆驼和绵羊）、流产胎儿样本（绵羊和山羊）以及人类的脑脊液和血液。这些分离株来自伊朗的13个省份，分离时间为2015年至2020年。根据多位点序列分型，ST8和ST2分别是羊种布鲁氏菌和牛种布鲁氏菌中最常见的序列类型。基于cgSNP分析的系统发育重建，所研究的伊朗布鲁氏菌菌株属于布鲁氏菌系统发育中的美洲和地中海谱系。此外，cgSNP分析显示伊朗布鲁氏菌分离株与伊拉克和埃及的菌株之间存在相似性。因此，可以认为伊朗菌株的起源是来自邻国和中东国家的菌株。此外，cgMLST分析表明，伊朗布鲁氏菌菌株与从伊拉克、阿富汗、叙利亚、土库曼斯坦和巴基斯坦的绵羊和人类中分离出的菌株密切相关。在当前的菌株组中，cgMLST和cgSNP分析为伊朗布鲁氏菌的有效溯源分析提供了合适且准确的工具。cgSNP和cgMLST的结果有助于了解伊朗菌株的地理分布和种间传播，并突出了在伊朗采用“同一健康”方法实施特定布鲁氏菌病控制措施的重要性。
