Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.
Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.
Front Cell Infect Microbiol. 2022 Dec 27;12:942328. doi: 10.3389/fcimb.2022.942328. eCollection 2022.
The crosstalk between the oral microbiome and oral cancer has yet to be characterized. This study recruited 218 patients for clinicopathological data analysis. Multiple types of specimens were collected from 27 patients for 16S rRNA gene sequencing, including 26 saliva, 16 swabs from the surface of tumor tissues, 16 adjacent normal tissues, 22 tumor outer tissue, 22 tumor inner tissues, and 10 lymph nodes. Clinicopathological data showed that the pathogenic bacteria could be frequently detected in the oral cavity of oral cancer patients, which was positively related to diabetes, later T stage of the tumor, and the presence of cervical lymphatic metastasis. Sequencing data revealed that compared with adjacent normal tissues, the microbiome of outer tumor tissues had a greater alpha diversity, with a larger proportion of , , and , while a smaller proportion of . The space-specific microbiome, comparing outer tumor tissues with inner tumor tissues, suggested minor differences in diversity. However, , , , and were more abundant in outer tumor tissues, while , , and were enriched in inner tumor tissues. Clinicopathology-specific microbiome analysis found that the diversity was markedly different between negative and positive extranodal extensions, whereas the diversity between different T-stages and N-stages was slightly different. and were enriched in T1/T2-stage patients and the non-lymphatic metastasis group, while and were enriched in the extranodal extension negative group. Taken together, high-throughput DNA sequencing in combination with clinicopathological features facilitated us to characterize special patterns of oral tumor microbiome in different disease developmental stages.
口腔微生物组与口腔癌之间的串扰尚未得到描述。本研究招募了 218 名患者进行临床病理数据分析。从 27 名患者收集了多种类型的标本进行 16S rRNA 基因测序，包括 26 份唾液、16 份肿瘤表面拭子、16 份相邻正常组织、22 份肿瘤外组织、22 份肿瘤内组织和 10 份淋巴结。临床病理数据显示，口腔癌患者口腔中可频繁检测到病原菌，与糖尿病、肿瘤晚期 T 分期和颈淋巴结转移的存在呈正相关。测序数据显示，与相邻正常组织相比，外肿瘤组织的微生物组具有更大的α多样性，具有更大比例的 、 、 和 ，而 比例较小。与内肿瘤组织相比，空间特异性微生物组提示外肿瘤组织的多样性差异较小。然而， 、 、 、和 在外肿瘤组织中更为丰富，而 、 、 和 在内肿瘤组织中更为丰富。特定于临床病理的微生物组分析发现，阴性和阳性结外延伸之间的多样性差异显著，而不同 T 分期和 N 分期之间的多样性差异较小。 、 和 在 T1/T2 期患者和非淋巴转移组中丰富，而 、 和 在无结外延伸组中丰富。总之，高通量 DNA 测序结合临床病理特征有助于我们在不同疾病发展阶段描述口腔肿瘤微生物组的特殊模式。
