Department of Radiation Oncology, The Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital, Yunnan Cancer Center), 519 Kunzhou Road, Xishan District, Kunming, 650118, China.
Department of Radiology, The Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital, Yunnan Cancer Center), 519 Kunzhou Road, Xishan District, Kunming, 650118, China.
BMC Cancer. 2024 Sep 17;24(1):1154. doi: 10.1186/s12885-024-12915-1.
The aim of this study was to characterize the microbiome of multiple mucosal organs in cervical cancer (CC) patients.
We collected oral, gut, urinary tract, and vaginal samples from enrolled study participants, as well as tumor tissue from CC patients. The microbiota of different mucosal organs was identified by 16S rDNA sequencing and correlated with clinical-pathological characteristics of cervical cancer cases.
Compared with controls, CC patients had reduced α-diversity of oral and gut microbiota (p < 0.001, p = 0.049, p = 0.013 p = 0.030), although there was an opposite trend in the vaginal microbiota (p = 0.028, p = 0.006). There were also significant differences in the β-diversity of the microbiota at each site between cases and controls (p = 0.002, p = 0.037, p = 0.001, p = 0.001). The uniformity of urine microbiota was lower in patients with cervical squamous cell carcinoma (p = 0.036) and lymph node metastasis (p = 0.027, p = 0.028, p = 0.021, p = 0.047). The composition of bacteria in urine also varied among patients with different ages (p = 0.002), tumor stages (p = 0.001) and lymph node metastasis (p = 0.002). In CC cases, Pseudomonas were significantly enriched in the oral, gut, and urinary tract samples. In addition, Gardnerella, Anaerococcus, and Prevotella were biomarkers of urinary tract microbiota; Abiotrophia and Lautropia were obviously enriched in the oral microbiota. The microbiota of tumor tissue correlated with other mucosal organs (except the gut), with a shift in the microflora between mucosal organs and tumors.
Our study not only revealed differences in the composition and diversity of the vaginal and gut microflora between CC cases and controls, but also showed dysbiosis of the oral cavity and urethra in cervical cancer cases.
本研究旨在分析宫颈癌（CC）患者多个黏膜器官的微生物组特征。
我们收集了入组研究参与者的口腔、肠道、尿路和阴道样本，以及 CC 患者的肿瘤组织。通过 16S rDNA 测序鉴定不同黏膜器官的微生物群，并将其与宫颈癌病例的临床病理特征相关联。
与对照组相比，CC 患者的口腔和肠道微生物多样性降低（p < 0.001，p = 0.049，p = 0.013，p = 0.030），而阴道微生物群则呈现相反的趋势（p = 0.028，p = 0.006）。病例组与对照组之间各部位微生物群的β多样性也存在显著差异（p = 0.002，p = 0.037，p = 0.001，p = 0.001）。宫颈鳞状细胞癌（p = 0.036）和淋巴结转移（p = 0.027，p = 0.028，p = 0.021，p = 0.047）患者的尿液微生物群均匀度较低。不同年龄（p = 0.002）、肿瘤分期（p = 0.001）和淋巴结转移（p = 0.002）的患者尿液中细菌的组成也有所不同。在 CC 病例中，假单胞菌在口腔、肠道和尿路上显著富集。此外，加德纳菌、厌氧球菌和普雷沃氏菌是尿路微生物群的生物标志物；口腔微生物群中明显富集了不动杆菌和洛特氏菌。肿瘤组织的微生物群与其他黏膜器官（肠道除外）相关，黏膜器官与肿瘤之间的微生物群发生了转移。
本研究不仅揭示了 CC 病例与对照组之间阴道和肠道微生物群组成和多样性的差异，还显示了宫颈癌患者口腔和尿道的菌群失调。
