Tizhe Emmanuel Vandi, Igbokwe Ikechukwu Onyebuchi, Njokwu Celestine Onwu-Ibe, Fatihu Mohammed Yakasai, Tizhe Ussa Delia, Ibrahim Najume Dogon-Giginya
Department of Veterinary Microbiology and Pathology, Faculty of Veterinary Medicine, University of Jos, Jos, Plateau State, Nigeria.
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Borno State, Nigeria.
Clin Pathol. 2024 Jul 26;17:2632010X241265854. doi: 10.1177/2632010X241265854. eCollection 2024 Jan-Dec.
The study was carried out to assess the effect of zinc supplementation on changes in calcium homeostasis, and parathyroid gland, bone, and skeletal muscle histology in rats exposed to subchronic oral glyphosate-based herbicide (GBH, GOBARA) toxicity.
Sixty male Wistar rats in 6 equal groups (DW, Z, G1, G2, ZG1, ZG2) were used: DW and Z were given 2 mL/kg distilled water and 50 mg/kg of zinc chloride (2%), respectively; G1 and G2 received 187.5 mg/kg and 375 mg/kg of glyphosate (in GBH), respectively; ZG1 and ZG2 were pretreated with 50 mg/kg of zinc chloride before receiving glyphosate, 1 hour later, at 187.5 and 375 mg/kg, respectively. Treatments were by gavage once daily for 16 weeks. Serum calcium, vitamin D, and parathormone were estimated. Histopathological examination of parathyroid gland, femoral bone and biceps femoris muscle was done.
GBH exposure caused significant ( = .0038) decrease in serum calcium concentration in G1, significant ( = .0337) decrease in serum vitamin D concentration in G1, significant increases in parathormone in G1 ( = .0168) and G2 ( = .0079) compared to DW. Significant ( > .05) changes did not occur in the other parameters of G2 compared to DW. Dose-dependent effect in GBH exposure was not observed after comparing G1 and G2. Necrotic changes occurred in parathyroid gland cells, osteocytes, and muscle cells in G1 and G2. In ZG1 and ZG2, significant ( > .05) variations in the parameters were not observed and tissue lesions were absent.
Subchronic GBH exposure impaired calcium homeostasis observed as hypocalcemia, hypovitaminemia D, and secondary hyperparathyroidism and caused tissue damage in parathyroid gland, bone, and muscle of rats and these were mitigated by zinc chloride pretreatment.
本研究旨在评估补充锌对亚慢性经口暴露于草甘膦基除草剂（GBH，GOBARA）毒性的大鼠钙稳态、甲状旁腺、骨骼和骨骼肌组织学变化的影响。
将60只雄性Wistar大鼠分为6个相等的组（DW、Z、G1、G2、ZG1、ZG2）：DW组和Z组分别给予2 mL/kg蒸馏水和50 mg/kg氯化锌（2%）；G1组和G2组分别接受187.5 mg/kg和375 mg/kg草甘膦（GBH中）；ZG1组和ZG2组在接受草甘膦前1小时分别用50 mg/kg氯化锌预处理，随后分别接受187.5 mg/kg和375 mg/kg草甘膦。每天经口灌胃给药一次，持续16周。检测血清钙、维生素D和甲状旁腺激素水平。对甲状旁腺、股骨和股二头肌进行组织病理学检查。
与DW组相比，G1组血清钙浓度显著降低（=0.0038），G1组血清维生素D浓度显著降低（=0.0337），G1组（=0.0168）和G2组（=0.0079）甲状旁腺激素显著升高。与DW组相比，G2组其他参数未发生显著（>0.05）变化。比较G1组和G2组后未观察到GBH暴露的剂量依赖性效应。G1组和G2组甲状旁腺细胞、骨细胞和肌肉细胞出现坏死变化。在ZG1组和ZG2组中，未观察到参数的显著（>0.05）变化，且无组织损伤。
亚慢性GBH暴露导致大鼠出现低钙血症、低维生素D血症和继发性甲状旁腺功能亢进，从而损害钙稳态，并导致甲状旁腺、骨骼和肌肉组织损伤，而氯化锌预处理可减轻这些损伤。
