Rydberg B
Mutat Res. 1978 Oct;52(1):11-24. doi: 10.1016/0027-5107(78)90091-x.
A screening procedure based on the formation of papillae on individual bacterial colonies was used to isolate mutants of Escherichia coli with high mutation rates in the presence of bromouracil. Most of the mutants obtained had high spontaneous mutation rates and mapped close to the previously known mutators mutT, mutS, mutR, uvrE and mutL. Except for mutants of mutT type, these mutators also showed high mutability by bromouracil. Transfection experiments were performed with heteroduplex lambda DNA to test for mismatch repair. The results suggest a reduced efficiency of repair of mismatched bases in mutators mutS, mutR, uvrE and mutL, whereas mutants mapping as mutT appear normal. The results support a connection between spontaneous and bromouracil-induced mutability and repair of mismatched bases in DNA.
一种基于在单个细菌菌落上形成乳头状物的筛选程序被用于在存在溴尿嘧啶的情况下分离具有高突变率的大肠杆菌突变体。获得的大多数突变体具有高自发突变率，并且定位在与先前已知的诱变基因mutT、mutS、mutR、uvrE和mutL附近。除了mutT类型的突变体，这些诱变基因在溴尿嘧啶存在下也表现出高突变性。用异源双链λDNA进行转染实验以测试错配修复。结果表明，诱变基因mutS、mutR、uvrE和mutL中错配碱基的修复效率降低，而定位为mutT的突变体似乎正常。这些结果支持了自发突变和溴尿嘧啶诱导的突变性与DNA中错配碱基修复之间的联系。
