清黛有效成分治疗溃疡性结肠炎的疗效：基于网络药理学的评价。

Efficacy of active ingredients in Qingdai on ulcerative colitis: a network pharmacology-based evaluation.

机构信息

Department of orthopedics, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.

The First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.

出版信息

J Tradit Chin Med. 2023 Feb;43(1):124-133. doi: 10.19852/j.cnki.jtcm.2023.01.011.

DOI:10.19852/j.cnki.jtcm.2023.01.011

PMID:36640003

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9924692/

Abstract

OBJECTIVE

To elucidate the protective effect of Qingdai (, QD) on ulcerative colitis (UC) by means of and approaches.

METHODS

A systems pharmacology analysis was per-formed to predict the active components of QD whereas the putative biological targets of QD against UC were obtained through target fishing, network cons-truction and enrichment analyses. Meanwhile, we examined the ameliorative effect of QD in a mouse model of dextran sulfate sodium (DSS)-induced colitis. During the 10-day experiment, the control and diseased mice were given with oral gavages of QD (1.3 g raw herbs·kg·d) or 5-aminosalicylic acid (5-ASA, 100 mg·kg·d) every day. The underlying pharma-cological mechanisms of QD in UC were determined using polymerase chain reaction tests, histological staining, enzyme-linked immunoassays, and Western blotting analysis.

RESULTS

Searching from various network pharmacology databases, 29 compounds were identified in QD. According to the screening criteria suggested by TCMSP (i.e. OB ≥ 30% and DL ≥ 0.18), nine of them were considered the active ingredients that contribute to the ameliorative effects of QD on different mouse models of colitis. Most importantly, the protective effect of QD on DSS-induced colitis was significantly associated with modulations of the expression levels of glycogen synthase kinase 3-β (Gsk3-β) and forkhead box p3 (Foxp3), which are widely considered as important regulators of excessive inflammatory responses.

CONCLUSIONS

The results of this study provide solid scientific evidence for the use of QD or its core active components in the clinical management of UC.

摘要

目的

通过系统药理学分析阐明清黛（QD）对溃疡性结肠炎（UC）的保护作用。

方法

通过靶标捕捞、网络构建和富集分析，预测 QD 的活性成分，获得 QD 针对 UC 的假定生物靶标。同时，我们在葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型中检验了 QD 的改善作用。在 10 天的实验中，对照组和疾病组每天口服给予 QD（1.3 克生药·kg·d）或 5-氨基水杨酸（5-ASA，100mg·kg·d）。采用聚合酶链反应试验、组织学染色、酶联免疫吸附试验和 Western blot 分析，确定 QD 在 UC 中的潜在药理机制。

结果

从各种网络药理学数据库中搜索，鉴定出 QD 中的 29 种化合物。根据 TCMSP（即 OB≥30%且 DL≥0.18）的筛选标准，其中 9 种被认为是 QD 对不同结肠炎小鼠模型具有改善作用的活性成分。最重要的是，QD 对 DSS 诱导的结肠炎的保护作用与糖原合成酶激酶 3-β（Gsk3-β）和叉头框 p3（Foxp3）表达水平的调节显著相关，这两者被广泛认为是过度炎症反应的重要调节因子。