Nie Jing, Liu Qin, Huang Shihui, Wang Jiafu, Niu Xi, Ran Xueqin
College of Animal Science, Guizhou University, Guiyang, Guizhou, China.
Institute of Agro-Bioengineering, Guizhou University, Guiyang, Guizhou, China.
Front Microbiol. 2025 Mar 4;16:1508280. doi: 10.3389/fmicb.2025.1508280. eCollection 2025.
Young rabbits are sensitive to surrounding changes and conditioned pathogens in intestine which might result in slow inflammation and diarrhea after microbial invasion. Traditional medicine herbs could provide efficacious treatment on slow infection and inflammation. The present research designed an (ACS) formula consisted of five types of Chinese herbs including (Rupr & Maxim) Harms (Ciwujia in Chinese), (Fisch) Bge (Huangqi in Chinese), (Qingdai in Chinese), Thunb (Yuxingcao in Chinese), and et Rhizoma (Gancao in Chinese). The effects of ACS decoction supplement were investigated via determination of cytokines and growth performances of young rabbits, and the flora in intestinal digesta from six fragments were further explored using 16S rRNA gene sequencing technology. Compared to the control group, rabbits supplied with different doses of ACS decoction possessed lower diarrhea and death rates, together with the IL-10 concentration, while the declined IL-1β and IL-12 levels and inflammatory factor gene expressions in intestinal tissues. Additionally, ACS addition changed the diversity of flora in each segments of intestine. Functional prediction on abundances of genera enriched to seven KEGG immunity pathways. Moreover, strong correlations were determined between the abundance of bacteria with interleukins contents, and the predictive immune signaling abundances, respectively. Especially, ACS exhibited anti-inflammation effects via decreasing the abundances of , , , and in intestine of young rabbits. In conclusion, dietary supplement with ACS exerted diarrhea-reducing effects, and improved immunity homeostasis by modulating intestinal flora diversity in young rabbits.
幼兔对周围环境变化和肠道中的条件致病菌敏感，微生物入侵后可能导致炎症反应缓慢和腹泻。传统草药可有效治疗慢性感染和炎症。本研究设计了一种由五种中药组成的复方（ACS），包括刺五加（Rupr & Maxim）Harms（中文名为刺五加）、黄芪（Fisch）Bge（中文名为黄芪）、青黛（中文名为青黛）、鱼腥草（Thunb）（中文名为鱼腥草）和甘草（et Rhizoma）（中文名为甘草）。通过测定幼兔的细胞因子和生长性能，研究了ACS水煎剂补充剂的效果，并使用16S rRNA基因测序技术进一步探索了来自六个片段的肠道消化物中的菌群。与对照组相比，饲喂不同剂量ACS水煎剂的兔子腹泻率和死亡率较低，IL-10浓度也较低，同时肠道组织中IL-1β和IL-12水平以及炎症因子基因表达下降。此外，添加ACS改变了肠道各段的菌群多样性。对富集到七个KEGG免疫途径的属丰度进行功能预测。此外，分别确定了细菌丰度与白细胞介素含量以及预测的免疫信号丰度之间的强相关性。特别是，ACS通过降低幼兔肠道中、、、和的丰度发挥抗炎作用。总之，日粮中添加ACS具有减少腹泻的作用，并通过调节幼兔肠道菌群多样性改善免疫稳态。
