School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, PR China.
College of Life Science, Shandong Agricultural University, Taian 271018, PR China.
Int J Biol Macromol. 2023 Mar 1;230:123241. doi: 10.1016/j.ijbiomac.2023.123241. Epub 2023 Jan 11.
The relationship between gut microbiota and type 2 diabetes mellitus (T2DM) has attracted increasing attention. In our work, one purified fraction a (AEPSa) was obtained from Cordyceps militaris polysaccharides, and its hypoglycemic activity and underlying mechanisms were investigated in high-fat diet (HFD)- and streptozotocin (STZ)-induced T2DM mice. The results revealed that AEPSa reshaped gut microbiota by increasing Allobaculum, Alistipes, Lachnospiraceae_NK4A136_group and norank_f_Muribaculaceae and decreasing Enterococcus and Ruminococcus_torques_group to inhibit the colonic toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) pathway and upregulate intestinal tight junction protein expression, thereby improving glucose and serum lipid metabolism, hormone secretion and complications. Fecal microbiota transplantation (FMT) also confirmed these findings. These results indicated that symptomatic relief of T2DM might be related to AEPSa regulating the gut microbiota against the TLR4/NF-κB pathway to protect the intestinal barrier. Therefore, AEPSa might be developed as a prebiotic agent against T2DM by regulating gut microbiota.
肠道微生物群与 2 型糖尿病（T2DM）之间的关系引起了越来越多的关注。在我们的工作中，从蛹虫草多糖中获得了一个纯化片段 a（AEPSa），并研究了其在高脂肪饮食（HFD）和链脲佐菌素（STZ）诱导的 T2DM 小鼠中的降血糖活性及其潜在机制。结果表明，AEPSa 通过增加 Allobaculum、Alistipes、Lachnospiraceae_NK4A136_group 和 norank_f_Muribaculaceae 并减少 Enterococcus 和 Ruminococcus_torques_group 来重塑肠道微生物群，从而抑制结肠 toll 样受体 4（TLR4）/核因子 kappa-B（NF-κB）途径并上调肠道紧密连接蛋白表达，从而改善葡萄糖和血清脂质代谢、激素分泌和并发症。粪便微生物群移植（FMT）也证实了这些发现。这些结果表明，T2DM 的症状缓解可能与 AEPSa 通过调节肠道微生物群对抗 TLR4/NF-κB 途径来保护肠道屏障有关。因此，AEPSa 可能通过调节肠道微生物群成为一种针对 T2DM 的益生元。
