Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, Jilin, China; College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin, China.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, Jilin, China.
Int J Biol Macromol. 2023 May 31;238:124333. doi: 10.1016/j.ijbiomac.2023.124333. Epub 2023 Apr 6.
Polysaccharides, as one of the main types of bioactive components of Cordyceps militaris, have anti-allergic asthma effects. Herein, an ovalbumin-induced allergic asthma mouse model was established to assess the potential mechanisms of the separated and purified Cordyceps militaris polysaccharide (CMP). CMP is an α-pyranose with a molecular weight of 15.94 kDa that consists of Glc, Man, Gal, Xyl, Ara and GlcA in a molar ratio of 81.25:21.96:13.88:3.92:3.58:1.00. CMP improved inflammatory cytokine levels, alleviated the histopathological changes in the lung and intestinal tissues, regulated the expression of mRNA and proteins related to oxidative stress and inflammatory pathways, reversed gut dysbiosis at the phylum and family levels and improved microbiota function in allergic asthma mice. Moreover, it was found that the levels of inflammatory cytokines in lung tissue of mice were significantly correlated with some intestinal microbial communities. Overall, CMP improved oxidative stress and the inflammatory response in allergic asthma mice by regulating the Nrf2/HO-1 and NF-κB signaling pathways, which may be closely correlation with maintaining the stability of the gut microbiota.
多糖作为蛹虫草主要的生物活性成分之一，具有抗过敏性哮喘的作用。本文建立了卵清蛋白诱导的过敏性哮喘小鼠模型，以评估分离纯化的蛹虫草多糖（CMP）的潜在作用机制。CMP 是一种α-吡喃糖，分子量为 15.94 kDa，由 Glc、Man、Gal、Xyl、Ara 和 GlcA 以 81.25:21.96:13.88:3.92:3.58:1.00 的摩尔比组成。CMP 可提高炎症细胞因子水平，减轻肺部和肠道组织的组织病理学变化，调节与氧化应激和炎症途径相关的 mRNA 和蛋白表达，逆转过敏哮喘小鼠在门和科水平上的肠道菌群失调，并改善其肠道微生物功能。此外，研究发现，小鼠肺部组织中炎症细胞因子的水平与某些肠道微生物群落显著相关。总之，CMP 通过调节 Nrf2/HO-1 和 NF-κB 信号通路，改善过敏性哮喘小鼠的氧化应激和炎症反应，这可能与维持肠道微生物群的稳定性密切相关。
