Fu Mengqi, Gao Xin, Xie Zuorui, Xia Chenlan, Gu Qing, Li Ping
Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
Foods. 2024 May 15;13(10):1539. doi: 10.3390/foods13101539.
Aging is characterized by the progressive degeneration of bodily tissues and decline in physiological functions, a process that may be exacerbated by imbalances in intestinal flora. Soluble dietary fiber (PSDF) from peel has demonstrated strong free radical scavenging ability to regulate intestinal flora in vitro. However, further evidence is required to ascertain the effectiveness of PSDF in vivo. In our study, 8-week-old mice were artificially aged through subcutaneous injections of a 200 mg/kg/d D-galactose solution for 42 days, followed by a 28-day dietary intervention with varying doses of PSDF, insoluble dietary fiber (PIDF), and vitamin C. After the intervention, we observed a significant mitigation of D-galactose-induced oxidative stress, as evident by weight normalization and reduced oxidative damage. 16S rRNA gene sequencing revealed that PSDF significantly altered the composition of intestinal flora, increasing Firmicutes and reducing Bacteroidota percentages, while also enriching colonic short-chain fatty acids (SCFAs). Spearman correlation analysis further identified a positive correlation between Firmicutes and isovaleric acid, and negative correlations between and acetic acid, and between and caproic acid. These findings support the potential of Citrus PSDF to alleviate oxidative stress.
衰老的特征是身体组织的渐进性退化和生理功能的下降，肠道菌群失衡可能会加剧这一过程。果皮中的可溶性膳食纤维（PSDF）在体外已显示出强大的自由基清除能力，可调节肠道菌群。然而，需要进一步的证据来确定PSDF在体内的有效性。在我们的研究中，8周龄的小鼠通过皮下注射200 mg/kg/d的D-半乳糖溶液进行人工老化42天，随后用不同剂量的PSDF、不溶性膳食纤维（PIDF）和维生素C进行28天的饮食干预。干预后，我们观察到D-半乳糖诱导的氧化应激显著减轻，体重恢复正常和氧化损伤减少证明了这一点。16S rRNA基因测序显示，PSDF显著改变了肠道菌群的组成，增加了厚壁菌门的比例，降低了拟杆菌门的比例，同时还丰富了结肠短链脂肪酸（SCFAs）。Spearman相关性分析进一步确定了厚壁菌门与异戊酸之间呈正相关，与乙酸之间以及与己酸之间呈负相关。这些发现支持了柑橘PSDF减轻氧化应激的潜力。
