Smeets W, Pauwels R, Laarakkers L, Debruyne F, Geraedts J
Stichting Ziekenhuisapotheek en Klinisch Laboratorium, Venray, The Netherlands.
Cancer Genet Cytogenet. 1987 Nov;29(1):23-7. doi: 10.1016/0165-4608(87)90027-6.
Twenty-seven successive bladder cancer tissue specimens, varying from noninfiltrating to deeply infiltrating ones, were chromosomally analyzed using a new direct method. Recognizable metaphases were obtained from 25 specimens (93%). In nearly all cases, suitable C-banding and in nine cases G-banding was achieved. Essential steps in this method are colcemid application in two steps, interrupted by washing with Hanks' balanced sodium solution and two separated hypotonic treatments. This method is easy to use and not time consuming.
使用一种新的直接方法对27个连续的膀胱癌组织标本进行了染色体分析，这些标本从非浸润性到深度浸润性不等。从25个标本（93%）中获得了可识别的中期分裂相。几乎在所有情况下，都实现了合适的C带染色，在9个病例中实现了G带染色。该方法的关键步骤是分两步应用秋水仙酰胺，中间用汉克斯平衡盐溶液冲洗，并进行两次分开的低渗处理。这种方法易于使用且不耗时。
