Smeets W, Pauwels R, Geraedts J
Cancer Genet Cytogenet. 1985 Apr 1;16(3):259-68. doi: 10.1016/0165-4608(85)90053-6.
Of 77 patients with bladder carcinoma, 99 tissue specimens--including tissues of patients with recurrent tumors taken after radiotherapy or cytostatics--were subjected to chromosomal analysis. In 42 specimens, recognizable metaphases could be obtained after conventional Giemsa staining and in a smaller number after C- and/or G-banding. All except one had abnormalities of the chromosomes. Short-term cultures for 24-48 hr in RPMI 1640 plus 15% fetal calf serum plus penicillin-streptomycin gave better results than a direct technique (30 min in 0.075 M KCl + 0.1 microgram colcemid/ml at 37 degrees C, followed by fixation). In low stage/grade tumors the number of recognizable metaphases obtained after short-term cultures is lower than in higher stage/grade tissue specimens.
在77例膀胱癌患者中，99份组织标本（包括放疗或使用细胞抑制剂后复发肿瘤患者的组织）接受了染色体分析。在42份标本中，经传统吉姆萨染色后可获得可识别的中期分裂相，少数标本经C带和/或G带染色后也可获得。除1例外，所有标本均有染色体异常。在RPMI 1640培养基中加入15%胎牛血清和青霉素-链霉素进行24至48小时的短期培养，比直接技术（在37℃下于0.075 M KCl + 0.1微克秋水仙酰胺/毫升中处理30分钟，随后固定）效果更好。在低分期/低分级肿瘤中，短期培养后获得的可识别中期分裂相数量低于高分期/高分级组织标本。
