Tokunaga K, Nakamura Y, Sakata K, Fujimori K, Ohkubo M, Sawada K, Sakiyama S
Division of Biochemistry, Chiba Cancer Center Research Institute and Hospital, Japan.
Cancer Res. 1987 Nov 1;47(21):5616-9.
We have shown that a Mr 37,000 protein whose expression is strongly enhanced in human lung cancer tissues is the subunit of glyceraldehyde-3-phosphate (GAPDH, EC 1.2.1.12). We have isolated a GAPDH complementary DNA from human lung cancer cells and deduced the complete amino acid sequence of the encoded protein. The structure of the lung cancer GAPDH is identical to that of liver GAPDH. In addition, we have found that GAPDH mRNA expression is markedly increased in human lung cancer tissues. These results disclose a molecular basis of increased glycolysis in cancer cells and reveal an important role of energy creating reaction in cancer cell growth.
我们已经证明，一种在人肺癌组织中表达强烈增强的37000分子量蛋白质是甘油醛-3-磷酸（GAPDH，EC 1.2.1.12）的亚基。我们从人肺癌细胞中分离出了GAPDH互补DNA，并推导了编码蛋白的完整氨基酸序列。肺癌GAPDH的结构与肝GAPDH的结构相同。此外，我们还发现GAPDH mRNA在人肺癌组织中的表达明显增加。这些结果揭示了癌细胞中糖酵解增加的分子基础，并揭示了能量产生反应在癌细胞生长中的重要作用。
