College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing 100871, China.
CCCC SINOBIOWAY E&P CO.,LTD, Jinan 250000, China.
Water Res. 2023 Mar 1;231:119589. doi: 10.1016/j.watres.2023.119589. Epub 2023 Jan 7.
Metabolic cross-feeding, in which species use metabolites of other members to promote their own growth, is vital for bacterial growth and survival. Thus, whether the unculturable bacteria can be isolated or purified from consortia by adding these essential metabolites remains elusive. In this study, mass spectrometry imaging vividly pictured symbionts supplied folate and gluconate to anammox bacteria to support their growth. After dosing folate and gluconate, the relative abundance and activity of anammox bacteria were substantially improved. Such enhancement is originated from the added folate and gluconate significantly eased metabolic burden of anammox bacteria as they no longer secreted the extracellular public goods to others for "resource exchange" during cross-feedings. On the other hand, the decreased supplement of extracellular "public goods" lead to the decay of symbionts with high demand for these metabolites in the consortia. This also deservedly increased the relative abundance of anammox bacteria. This study provides a new dimension to isolate specific functional bacteria based on metabolic cross-feedings.
代谢交叉喂养，即物种利用其他成员的代谢物来促进自身生长，对细菌的生长和存活至关重要。因此，通过添加这些必需的代谢物，能否从共生体中分离或纯化不可培养的细菌仍然难以捉摸。在这项研究中，质谱成像生动地描绘了共生体向厌氧氨氧化菌提供叶酸和葡萄糖酸，以支持其生长。添加叶酸和葡萄糖酸后，厌氧氨氧化菌的相对丰度和活性得到了显著提高。这种增强源于添加的叶酸和葡萄糖酸显著减轻了厌氧氨氧化菌的代谢负担，因为它们不再分泌细胞外公共物品进行“资源交换”，从而促进了代谢交叉喂养。另一方面，细胞外“公共物品”的减少导致对这些代谢物需求较高的共生体中的共生体的衰退。这也理所当然地增加了厌氧氨氧化菌的相对丰度。这项研究为基于代谢交叉喂养分离特定功能细菌提供了一个新的维度。
