College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing, 100871, China.
College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Water Res. 2021 Mar 1;191:116802. doi: 10.1016/j.watres.2020.116802. Epub 2021 Jan 2.
Both the benefits of bacterial quorum sensing (QS) and cross-feeding for bio-reactor performance in wastewater treatment have been recently reported. As the social traits of microbial communities, how bacterial QS regulating bacterial trade-off by cross-feeding remains unclear. Here, we find diffusion signal factor (DSF), a kind of QS molecules, can bridge bacterial interactions through regulating public goods (extracellular polymeric substances (EPS), amino acids) for metabolic cross-feedings. It showed that exogenous DSF-addition leads to change of public goods level and community structure dynamics in the anammox consortia. Approaches involving meta-omics clarified that anammox and a Lautropia-affiliated species in the phylum Proteobacteria can supply costly public goods for DSF-Secretor species via secondary messenger c-di-GMP regulator (Clp) after sensing DSF. Meanwhile, DSF-Secretor species help anammox bacteria scavenge extracellular detritus, which creates a more suitable environment for the anammox species, enhances the anammox activity, and improves the nitrogen removal rate of anammox reactor. The trade-off induces discrepant metabolic loads of different microbial clusters, which were responsible for the community succession. It illustrated the potential to artificially alleviate metabolic loads for certain bacteria. Deciphering microbial interactions via QS not only provides insights for understanding the social behavior of microbial community, but also creates new thought for enhancing treatment performance through regulating bacterial social traits via quorum sensing-mediated public goods.
最近有报道称，细菌群体感应 (QS) 和交叉喂养对废水处理中生物反应器性能的好处。作为微生物群落的社会特征，细菌 QS 通过交叉喂养调节细菌权衡的方式仍不清楚。在这里，我们发现扩散信号因子 (DSF)，一种 QS 分子，可以通过调节公共物品（细胞外聚合物物质 (EPS)、氨基酸）进行代谢交叉喂养来桥接细菌相互作用。结果表明，外源性 DSF 的添加会导致氨氮单胞菌联合体中公共物品水平和群落结构动态的变化。涉及元组学的方法阐明，氨氮和变形菌门中与 Lautropia 相关的物种可以通过二次信使 c-di-GMP 调节剂 (Clp) 感知 DSF 后为 DSF-Secretor 物种提供昂贵的公共物品。同时，DSF-Secretor 物种帮助氨氮细菌清除细胞外碎屑，为氨氮物种创造更适宜的环境，提高氨氮活性，并提高氨氮反应器的脱氮率。这种权衡导致不同微生物群的代谢负荷不同，这是群落演替的原因。它说明了人为减轻某些细菌代谢负荷的潜力。通过 QS 破译微生物相互作用不仅为理解微生物群落的社会行为提供了思路，而且通过群体感应介导的公共物品调节细菌的社会特征为增强处理性能创造了新的思路。
