Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, China.
Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, China.
Fish Shellfish Immunol. 2023 Feb;133:108547. doi: 10.1016/j.fsi.2023.108547. Epub 2023 Jan 13.
NF-E2-related factor-like-2 (Nrf2) is a transcription factor that belongs to the Cap'n'Collar transcription factor family and plays a role in regulating inflammation, autophagy, metabolism, proteostasis, and cancer prevention. However, its influence on Vibrio spp infection in L. vannamei remains uncertain. In this study, the effects of Nrf2 on the immune response in Vibrio spp infection was determined by RT-PCR and histopathological analysis. The results showed that RNAi of Nrf2 significantly decreased the expression of antioxidant-related genes (CAT, SOD and GST; p < 0.05), and significantly up-regulated inflammation-related genes (IMD, pro-PO, P38, Toll, Hsp70, NFκB and RAB6A; p < 0.05) and the apoptosis gene (caspase3). Under the infection of V. harveyi, histopathological analysis showed that after RNAi of Nrf2, the hepatopancreas of shrimp has an abnormal arrangement of hepatic tubules and vacuolization of hepatocyte; The basement membrane is peeled off and the epithelial cells are massively necrotic. Compared with the RNAi of Nrf2 group, the tissue damage in the SFN group was much lessened, and there were fewer apoptosis signals in the TUNEL assay. In conclusion, this experiment indicated that Nrf2 is involved in the regulation of inflammatory response, oxidative stress,and apoptosis induced by V. harveyi in L. vannamei.
NF-E2 相关因子 2（Nrf2）是一种转录因子，属于 Cap'n'Collar 转录因子家族，在调节炎症、自噬、代谢、蛋白质稳态和癌症预防方面发挥作用。然而，它对凡纳滨对虾中弧菌感染的影响尚不确定。在这项研究中，通过 RT-PCR 和组织病理学分析确定了 Nrf2 对弧菌感染免疫反应的影响。结果表明，Nrf2 的 RNAi 显著降低了抗氧化相关基因（CAT、SOD 和 GST；p<0.05）的表达，并显著上调了炎症相关基因（IMD、pro-PO、P38、Toll、Hsp70、NFκB 和 RAB6A；p<0.05）和凋亡基因（caspase3）。在感染哈维弧菌后，组织病理学分析表明，Nrf2 的 RNAi 后，虾的肝胰腺中肝小管排列异常，肝细胞出现空泡化；基膜脱落，上皮细胞大量坏死。与 Nrf2 RNAi 组相比，SFN 组的组织损伤明显减轻，TUNEL 检测到的凋亡信号也较少。总之，本实验表明 Nrf2 参与了凡纳滨对虾中哈维弧菌诱导的炎症反应、氧化应激和细胞凋亡的调节。
