Johnson P J, Kooter J M, Borst P
Division of Molecular Biology, Netherlands Cancer Institute, Antoni van Leeuwenhoekhuis, Amsterdam.
Cell. 1987 Oct 23;51(2):273-81. doi: 10.1016/0092-8674(87)90154-1.
We have used inactivation of transcription by UV irradiation to map transcription units in trypanosomes. The relative inactivation rate of the transcription of mini-exon, 5S, and rRNA genes was inversely proportional to the previously estimated lengths of these transcription units. The telomeric transcription unit containing the gene for variant-specific surface glycoprotein (VSG) 221 was inactivated as a single unit of 60 kb. This long transcription unit comprises at least one other protein-coding gene and yields seven other stable mRNAs. These data thus provide evidence for a multicistronic transcription unit for cellular genes in a eukaryote.
我们利用紫外线照射使转录失活来绘制锥虫中的转录单元图谱。小外显子、5S和rRNA基因转录的相对失活率与先前估计的这些转录单元的长度成反比。包含可变特异性表面糖蛋白（VSG）221基因的端粒转录单元作为一个60 kb的单一单元被失活。这个长转录单元至少包含另一个蛋白质编码基因，并产生另外七种稳定的mRNA。因此，这些数据为真核生物中细胞基因的多顺反子转录单元提供了证据。
