Reinhart R A, Marx J J, Haas R G, Desbiens N A
Department of Cardiology, Marshfield Clinic, WI 54449.
Clin Chim Acta. 1987 Aug 14;167(2):187-95. doi: 10.1016/0009-8981(87)90371-8.
Measurement of intracellular magnesium (Mg2+) may have advantages over serum Mg2+ measurements in the assessment of Mg2+ homeostasis in patients. An accurate and reproducible method of measuring the Mg2+ content of mononuclear cells (lymphocytes and monocytes) from the venous blood of human subjects utilizing commonly available reagents and equipment is described. A well-defined clinical population of 88 subjects (48 males and 40 females) was used as the normal population. The mean mononuclear cell Mg2+ content was 67.8 +/- 13.8 (SD) fg/cell giving a 95% confidence interval normal range of 40-95 fg/cell which compared favorably to the empirical 95% limits of 44-94 fg/cell. The serum Mg2+ measured at the same time was 0.90 +/- 0.06 (SD) mmol/l. No significant correlation was found when comparing serum and mononuclear cell Mg2+ concentrations. Additionally, no significant differences were found when serum and intracellular Mg2+ were analyzed for sex and age with the exception that intracellular Mg2+ tended to be slightly higher in the younger age groups. The routine performance of this assay may require as little as 7 ml of venous blood and was done with a coefficient of variation of 3.0-3.6%.
在评估患者的镁（Mg2+）稳态方面，测量细胞内镁（Mg2+）可能比测量血清镁（Mg2+）更具优势。本文描述了一种利用常用试剂和设备测量人体静脉血中单核细胞（淋巴细胞和单核细胞）镁（Mg2+）含量的准确且可重复的方法。以88名受试者（48名男性和40名女性）组成的明确临床群体作为正常人群。单核细胞镁（Mg2+）含量的平均值为67.8±13.8（标准差）fg/细胞，95%置信区间的正常范围为40 - 95 fg/细胞，与经验性的44 - 94 fg/细胞的95%限值相比具有优势。同时测量的血清镁（Mg2+）为0.90±0.06（标准差）mmol/L。比较血清和单核细胞镁（Mg2+）浓度时未发现显著相关性。此外，分析血清和细胞内镁（Mg2+）的性别和年龄差异时未发现显著差异，不过细胞内镁（Mg2+）在较年轻年龄组中往往略高。该检测方法常规操作可能只需7毫升静脉血，变异系数为3.0 - 3.6%。
